VITAMIN A IN GROWTH AND DEVELOPMENT

建议的体内和体外营养和营养的目标 生化研究是继续确定作用机制(S) 重要的微量营养素维生素A在器官中的分子水平 除了眼睛。两种维生素作用方式的参与 不同特异性胞浆结合蛋白的反应 (维生素A酒精)和维甲酸(维生素A酸)提纯到 来自大鼠肝脏和睾丸的同质性将受到特别关注。 体外研究提出了视黄醇和维甲酸是如何与其结合的 相应的结合蛋白与由此改变的细胞核相互作用 支持细胞分化的基因组表达。活体内 研究的目的是阐明调节细胞周期的因素。 这些结合蛋白在成人和新生儿各器官中的水平 老鼠。为此，为这两种结合开发了敏感的放射免疫系统 蛋白质将被利用。

The objective of proposed in vivo as well as in vitro nutritional and biochemical research is to continue to define the mechanisms(s) of action of the important micronutrient vitamin A at the molecular level in organs other than the eye. The participation in the mode of vitamin action of two distinct specific intracellular cytoplasmic binding proteins for retionl (vitamin A alcohol) and for retinoic acid (vitamin A acid) purified to homogeneity from rat liver and testis will receive particular attention. In vitro studies are proposed how retinol and retinoic acid bound to their respective binding proteins interact with the cell nucleus changing thereby the genomic expression underlying cell differentiation. The in vivo studies are directed towards the elucidation of factor which regulate the levels of these binding proteins in various organs in adult and in neonatal rats. To this end sensitive radioimmunoassys developed for both binding proteins will be utilized.