HUMAN CHORIONIC AND OTHER GONADOTROPINS

这项工作涉及的结构，功能和生物合成的研究， 促性腺激素 结构研究包括二硫键的归属 在人绒毛膜促性腺激素（hCG）的α和β亚基中， 绵羊和牛促黄体激素碳水化合物单位的结构 （o，bLH）和孕马血清促性腺激素（PMSG）。 所有的二硫键 在hCG-α和hCG-β中已经分配。 的碳水化合物结构 测定了oLH和bLH。 已经发现碳水化合物 它们结构模式与其他国家的结构模式大不相同 糖蛋白 对PMSG的碳水化合物结构的研究将是 启动。 结构和功能研究主要集中在 修饰hCG-β以获得适用于以下的高度特异性抗原 产生hCG特异性抗体。 此外，这些研究还包括 确定碳水化合物在hCG功能中的作用。 去除 从hCG中提取碳水化合物，产生了抑制 腺苷酸环化酶和类固醇生成。 衍生物的作用机制 会得到考验 生物合成研究涉及测定 寡糖-脂质中间体-碳水化合物前体结构 糖蛋白的单位。 最后，对作品进行了分离和表征 大鼠卵巢hCG/hLH受体的研究进展。

The work is related to studies on the structure, function and biosynthesis of gonadotropins. The structural studies include the assignment of disulfide bonds in the alpha and beta subunits of human chorionic gonadotropins (hCG), structures of the carbohydrate units of ovine and bovine luteinizing hormones (o, bLH) and pregnant mare serum gonadotropin (PMSG). All the disulfide bonds in hCG-alpha and hCG-beta have been assigned. The carbohydrate structures of oLH and bLH have been determined. It has been found that the carbohydrate structural pattern in them is quite different from that present in other glycoproteins. The work on the carbohydrate structure of PMSG will be initiated. The structure and function studies have focused primarily on the modification of hCG-beta to obtain a highly specific antigen suitable for producing an hCG specific antibody. Also, these studies have included the determination of the role of carbohydrate in the function of hCG. The removal of carbohydrate from hCG has resulted in the derivatives which are inhibitors of adenyl cyclase and steriodogenesis. The mechanism of action of the derivatives will be tested. The biosynthetic studies have involed the determination of the structure of oligosaccharide-lipid intermediate -a precursor of carbohydrate unit in glycoproteins. Finally, the work on the isolation and characterization of hCG/hLH receptor from rat ovaries is in progress.