ASSEMBLY OF VIRAL MEMBRANES

该项目的长期目标是了解细胞内 副粘病毒附着糖蛋白的加工和融合 糖蛋白 本申请的具体目的是：1. 以限定 纽卡斯尔HN和F糖蛋白的膜插入步骤 疾病病毒专注于膜插入所需的信号， 每个蛋白质的最终正确的膜拓扑结构，2. 以限定 两种蛋白质的细胞内加工集中在要求 蛋白质的正确折叠和参与的细胞机制 这个过程，3。 定义正确组装所需的序列 在细胞表面的HN蛋白质和进入病毒体。 中的步骤 膜插入将在无细胞翻译系统中表征 含有膜。 膜插入后的事件将 也可以在无细胞系统中进行检查。基因突变对 蛋白质的折叠和随后通过细胞的运输也将 可以通过分离基因改变的细胞来探索。 感染的细胞会 也可用于表征野生型蛋白质。 最后，一个补充 用改变的基因转染的细胞和病毒缺陷的细胞之间的系统 在该基因的表达中将允许确定序列 蛋白质正确组装成病毒体的要求。 这些研究有助于理解真核细胞的通路 用于糖蛋白的膜插入、运输和加工 梗概. 为这些蛋白质建立的原则将具有相关性 糖蛋白。 这些研究对一个 理解功能的结构决定因素 副粘病毒糖蛋白。 全面了解结构 功能的决定因素需要了解必要的步骤 以获得适当的结构。

The long-term goal of this project is to understand the intracellular processing of Paramyxovirus attachment glycoprotein and fusion glycoprotein. The specific aims of this application are: 1. To define the steps in membrane insertion of the HN and F glycoproteins of Newcastle disease virus focusing on the signals required for membrane insertion and the final correct membrane topology of each protein, 2. To define intracellular processing of the two proteins focusing on the requirements for proper folding of the proteins and the cellular machinery involved in this process, 3. To define the sequences required for the proper assembly of the HN protein at the cell surface and into virions. The steps in membrane insertion will be characterized in cell-free translation systems containing membranes. Events just subsequent to membrane insertion will also be examined in a cell-free system. The effect of mutations on the folding and subsequent transport of the protein through the cell will also be explored by transfecting cells with altered genes. Infected cells will also be used to characterize wild-type proteins. Lastly, a complementation system between cells transfected with an altered gene and virus defective in the expression of that gene will allow determination of the sequence requirements for proper assembly of the proteins into virions. These studies are relevant to an understanding of eucaryotic cell pathways used for the membrane insertion, transport and processing of glycoproteins in general. Principles established for these proteins will have relevance to glycoproteins in general. These studies are also important to an understanding of the structural determinants of the function of Paramyxovirus glycoproteins. A full understanding of the structural determinants of function requires an understanding of the steps necessary to acquire the proper structure.