Building long-read high-fidelity sequencing resources to support Bioscience Research in the UK

基因组学革命正在进入一个新的阶段，新的测序技术可以以前所未有的精度和低成本读取长DNA分子。这些高保真长的DNA序列读取可用于生成高质量的无间隙参考基因组，完全表征转录组，描述甲基组，以及表征肠道微生物组或土壤或水环境样品中复杂微生物混合物中的微生物。PacBio Revio系统是最新的长读DNA测序平台，可以以可承受的成本生成大量的长读数据。revo的一个关键特点是，它可以通过多次读取DNA分子来纠正错误，从而产生高度精确的长读。这笔拨款将获得苏格兰首个revo平台，并将其嵌入爱丁堡基因组公司，使我们能够与生物和生物技术研究界的研究人员合作，提供低成本、高质量的长读测序。我们将利用这笔投资来支持那些希望为农业、生物技术、遗传学、免疫学和合成生物学等不同主题生成基因组数据的研究人员。我们还将提供先进的基因组学数据培训，以支持revo在更广泛的用户群体中的应用。

The genomics revolution is entering a new phase, with new sequencing technologies that can read long DNA molecules with unprecedented accuracy and low costs. These high fidelity long DNA sequence reads can be used to generate high-quality gapless reference genomes, to fully characterise transcriptomes, to describe methylomes, and to characterize microorganisms in complex microbial mixtures such as in the gut microbiome or in environmental samples of soil or water. The PacBio Revio System is the latest long-read DNA sequencing platform that can generate extremely large amounts of long read data at an affordable cost. A key feature of the Revio is that it can generate highly accurate long reads, by reading a DNA molecule multiple times to correct errors. This grant will acquire the Revio platform, the first in Scotland, embed this within Edinburgh Genomics, and allow us to offer low-cost, high quality long read sequencing in collaboration with researchers in the biological and biotechnology research community. We will use the investment to support researchers who want to generate genomic data for diverse topics such as agriculture, biotechnology, genetics, immunology and synthetic biology. We will also offer advanced genomics data training to support the uptake of the Revio within the wider user community.