EFFECTORS OF FUNCTIONAL ORGANIZATION OF TRANSMEMBRANE

骨骼肌和心肌中的许多阳离子通道是跨膜的。 蛋白质。因此，它们包含用于识别 通道开关器信使以及用于开闭的调制 有机、一价阳离子和二价阳离子的状态。跨膜 蛋白质在脂质环境中包含其结构的一部分 其他在不同离子强度和组成的介质中和 在牢房外。阐明不同部分的作用 蛋白质分子对这个项目很有意义。建议的工作是 对于4个基本推力区：1)脂类或蛋白质构象的作用和 功能，2)单抗对蛋白质功能的影响，3) 特异性单抗结构位点的鉴定 通道功能的效应物和4)其他蛋白质的作用研究 膜上的状态与通道功能有关。拟议的工作将使用 乙酰胆碱受体作为阳离子肌肉通道的原型 有机阳离子和无机阳离子。在这些研究中，我们合成并获得了一种 蛋白质介导膜分子事件的自洽成像 使用以下技术的事件：1)物理工具、差异 扫描量热法、荧光动力学和傅里叶变换红外光谱， 2)动力学过程、平衡结合、离子通量速率、荧光 淬火停流程序，3)生物工具，膜 重建，单抗，成肌细胞培养和膜 分级，4)化学程序，多肽分级和分离， 多肽的化学表征，亲和配体标记， 还原甲基化、受控蛋白质分解、蛋白质化学。这些 不同的输入线路集成在一个实验室中 环境。

Many cationic channels in skeletal and heart muscle are transmembranous proteins. As such they contain structural elements for recognition of the channel opener messenger as well as for modulation of the open and close states for organic and monovalent and divalent cations. Transmembrane proteins contain portions of their structure in a lipid environment and others in media of different ionic strengths and composition inside and outside the cell. Elucidation of the roles of the different sections of the protein molecule is of interest to this project. The proposed work is for 4 basic thrust areas: 1) role of lipids or protein conformation and function, 2) effect of monoclonal antibodies on protein function, 3) identification of structural sites for specific monoclonal antibodies effectors of channels function and 4) study of effects of other protein membrane on states related to channel function. The proposed work will use the acetylcholine receptor as prototype of cationic muscle channel for organic and inorganic cations. In these studies we synthesize and obtain a self consistent image of molecular events for a protein mediated membrane event using the following techniques: 1) physical tools, differential scanning calorimetry, fluorescence dynamics and FT-infrared spectroscopy, 2) kinetic procedures, equilibrium binding, ion flux rates, fluorescence quenching stopped-flow procedures, 3) biological tools, membrane reconstitution, monoclonal antibodies, myoblasts cell culture and membrane fractionation, 4) chemical procedures, peptide fractionation and isolation, chemical characterization of peptides, labeling with affinity ligands, reductive methylation, controlled proteolysis, protein chemistry. These different lines of input are integrated within one single laboratory environment.