ATRESIA IN CYCLING

这个项目的总体目标是确定毛囊炎的原因。 闭锁在骑自行车大鼠。 将给予抗睾酮抗血清， 测试卵泡内雄激素的积累是 闭锁的直接原因 确定LH激增是否促进闭锁， 预先暴露于FSH可预防闭锁， 促性腺激素的激增将被解离。 猪卵泡液 （"FSH"）将用于减弱FSH激增;外源性FSH和LH将 施用以"促进"或增加内源性促性腺激素激增。 的 这些处理对小窦卵泡数量和活力的影响 （那些卵泡特别容易闭锁），将通过 组织学方法 将为以下序列建立时间过程： 与这些卵泡闭锁相关的形态学事件。 小窦状 将分离卵泡并置于组织培养中，以确定暴露是否 LH激增刺激雄激素的产生， 刺激芳香酶活性。

The overall goal of this project is to determine the causes of follicular atresia in the cycling rat. Anti-testosterone antiserum will be administered to test the hypothesis that accumulation of androgen within the follicle is the immediate cause of atresia. To determine if the LH surge promotes atresia, and prior exposure to FSH prevents atresia, the components of the periovulatory surge of gonadotropins will be dissociated. Porcine follicular fluid ("inhibin") will be used to attenuate the FSH surge; exogenous FSH and LH will be administered to "advance" or augment the endogenous gonadotropin surges. The effect of these treatments on the number and viability of small antral follicles (those follicles particularly prone to atresia), will be examined by histological methods. A time course will be established for the sequence of morphological events associated with atresia in these follicles. Small antral follicles will be isolated and placed in tissue culture to determine if exposure to the LH surge stimulates androgen production, and exposure to the FSH surge stimulates aromatase enzyme activity.