VITAMIN A IN GROWTH AND DEVELOPMENT

提出的体内和体外营养和 生物化学研究的目的是继续确定作用机制 重要的微量营养素维生素A在器官中的分子水平 除了眼睛。 参与维生素作用方式的两种 不同的特异性视网膜细胞内结合蛋白 （维生素A醇）和维甲酸（维生素A酸）纯化， 来自大鼠肝脏和睾丸的均一性将受到特别关注。 体外研究提出了视黄醇和视黄酸如何结合到它们的 相应的结合蛋白与细胞核相互作用， 细胞分化的基因组表达。 体内 研究的方向是阐明调节细胞凋亡的因子。 这些结合蛋白在成人和新生儿的各种器官中的水平 大鼠 为此，开发了灵敏的放射免疫分析法， 蛋白质将被利用。

The objective of proposed in vivo as well as in vitro nutritional and biochemical research is to continue to define the mechanisms(s) of action of the important micronutrient vitamin A at the molecular level in organs other than the eye. The participation in the mode of vitamin action of two distinct specific intracellular cytoplasmic binding proteins for retionl (vitamin A alcohol) and for retinoic acid (vitamin A acid) purified to homogeneity from rat liver and testis will receive particular attention. In vitro studies are proposed how retinol and retinoic acid bound to their respective binding proteins interact with the cell nucleus changing thereby the genomic expression underlying cell differentiation. The in vivo studies are directed towards the elucidation of factor which regulate the levels of these binding proteins in various organs in adult and in neonatal rats. To this end sensitive radioimmunoassys developed for both binding proteins will be utilized.