SPATIAL PATTERN OF THE EGG CHAMBER IN DROSOPHILA

这个项目的目的是分析空间不对称是如何在 果蝇发育中的卵室。我们已经证明了 生殖系和覆盖的毛囊细胞之间的信号传递过程 建立了卵壳和胚胎的背腹模式。 我们发现，鱼雷，这条细胞通讯途径的中心基因， 编码脊椎动物表皮生长因子的果蝇同源物 受体(EGFR)。我们将在分子水平上分析一组32个 鱼雷等位基因对受体功能的不同影响 单元类型。 分析鱼雷激活所涉及的上游生殖系过程 我们对Cornichon基因进行了详细的遗传和分子分析 它目前是我们编码该配体的最佳候选者 受体。我们随后将分析角鱼之间的相互作用 以及gurken、fs(1)K10、卡布奇诺和尖顶，这些基因也作用于 生殖系和参与配基生产。这将使我们能够 描述系统使用的时间和空间控制路径 配基生产。 我们将分析被激活的两条细胞反应通路 鱼雷受体：背部毛囊细胞命运的建立和 对一种新的、腹型信号的调节，这种信号是在 卵泡细胞，并传输回胚胎。我们已经确定了一个 第一反应途径的候选基因类别。我们建议 分析这一类并确定促进建立 背部毛囊细胞的命运。我们还发现了一种基因(Windbeutel) 它作用于第二条途径，并参与新的 最终建立胚胎细胞的卵泡细胞中的信号 命运。基因和分子技术将被用来分析 温德比特尔。此外，寻找其他作用于基因的基因。 鱼雷途径将通过筛选增强子或抑制子来执行 突变。 我们还将分析影响卵室早期事件的突变。 图案化。这将提供有关主要机制的信息 在果蝇中用来建立初始的前后部和 背腹不对称。 拟议的研究将使我们能够确定如何在空间上和 鱼雷受体酪氨酸激酶的时间受控激活是 ，并提供了对 受受体控制的不同发育途径 激活。关于这种物质作用方式的极好的生化数据 受体是利用脊椎动物组织培养系统获得的。我们的 对果蝇卵室的研究将补充这些分析，并且 深入了解这类受体在发育中的有机体中的作用。

The aim of this project is the analysis how spatial asymmetries arise in the developing egg chamber of Drosophila melanogaster. We have shown that a signalling process between the germline and the overlying follicle cells establishes the dorso-ventral pattern of both the egg shell and the embryo. We found that torpedo, a central gene in this cell communication pathway, encodes the Drosophila homolog of the vertebrate Epidermal Growth Factor receptor (EGFr). We will analyze at the molecular level a set of 32 torpedo alleles which differentially affect receptor function in different cell types. To analyze the upstream germline processes involved in torpedo activation we propose a detailed genetic and molecular analysis of the gene cornichon which is presently our best candidate for encoding a ligand of the receptor. We will subsequently analyze the interactions between cornichon and gurken, fs(1)K10, capuccino, and spire, genes that also act in the germline and are involved in ligand production. This will allow us to describe the pathway of temporal and spatial control used by the system for ligand production. We will analyze the two cellular response pathways that are activated by the torpedo receptor: the establishment of dorsal follicle cell fates and the regulation of a new, ventralizing signal which is produced in the follicle cells and transmitted back to the embryo. We have identified a candidate class of genes for the first response pathway. We propose to analyze this class and identify genes that promote the establishment of dorsal follicle cell fates. We have also identified a gene (windbeutel) that acts in the second pathway and participates in the production of a new signal in the follicle cells which ultimately established embryonic cell fates. Genetic and molecular techniques will be used to analyze windbeutel. In addition, a search for additional genes that act in the torpedo pathway will be performed by screening for enhancer or suppressor mutations. We will also analyze mutations that affect early events in egg chamber patterning. This will provide information about the primary mechanisms that are used in Drosophila to establish the initial anterior-posterior and dorso-ventral asymmetries. The proposed research will allow us to determine how a spatially and temporally controlled activation of the torpedo receptor tyrosine kinase is achieved in development, and provide a detailed understanding of the different developmental pathways that are controlled by receptor activation. Excellent biochemical data on the mode of action of such receptors has been obtained using vertebrate tissue culture systems. Our studies in the egg chamber of Drosophila will complement such analyses, and yield insights into the role of such receptors in the developing organism.