VITAMIN A IN GROWTH AND DEVELOPMENT

拟议的营养和生化研究的目标是 继续定义微量营养素作用的分子机制(S) 维生素A(视黄醇)及其代谢产物维甲酸在发育器官中的作用 除了眼睛。视黄醇和维甲酸与基因组的相互作用 将在围产期肺、肝和睾丸发育期间进行研究。 视黄醇缺乏期间基因表达的变化将 在饮食造成视黄醇缺乏的整个动物上进行研究 限制和重新喂食视黄醇或视黄酸后不久 (再饲喂模式)。在分析这种营养物质与 基因组，两种特异的视黄醇细胞内结合蛋白(CRABP)和 对于维甲酸(CRABP)，将受到特别关注。使用 再喂模型中，将探索基因编码是否以及如何编码 这些蛋白质受它们各自的配体和荷尔蒙的调节。 此外，还将研究这些蛋白质的基因是如何 围产期各器官发育过程中的特殊调节 强调肺与睾丸的分化。这些细胞表达 这些结合蛋白的基因将在肺和肺中被发现 睾丸组织。将尝试澄清组织特异性 视黄醇和视黄酸通过研究与视黄醇和视黄酸结合的蛋白质发挥作用 CRBP和CRABP基因启动子区域。最后给出了实验结果。 检测和鉴定视黄醇和视黄酸激活的早期基因 酸。

The objective of the proposed nutritional and biochemical research is to continue to define molecular mechanism(s) of action of micronutrient vitamin A (retinol) and its metabolite retinoic acid in developing organs other than the eye. Retinol and retinoic acid interactions with the genome will be studied during perinatal development of lungs, liver, and testes. Changes which occur in the gene expression during retinol deficiency will be studied in the whole animal made retinol deficient by dietary restriction and shortly after refeeding with retinol or retinoic acid (refeeding model). In the analysis of interaction of this nutrient with the genome, two specific intracellular binding proteins for retinol (CRABP) and for retinoic acid (CRABP) will receive particular attention. Using the refeeding model, it will be explored whether and how the genes coding for these proteins are regulated by their respective ligands and by hormones. Furthermore, it will be studied how genes for these proteins are being regulated during perinatal development of various organs with special emphasis on the lung and testicular differentiation. The cells expressing genes for these binding proteins will be identified in pulmonary and testicular tissue. It will be attempted to clarify the tissue specific action of retinol and retinoic acid by studying proteins binding to the promoter regions of CRBP and CRABP genes. Finally, experiments are proposed to detect and characterize early genes activated by retinol and retinoic acid.