INSTRUMENTATION FOR ANALYSIS OF HIGH MASS BIOMOLECULES

这项建议的长期目标是为 一种相对较小、便宜但非常强大的工具，用于获得 高质量的结构信息和相对分子质量 生物分子。具体地说，该仪器将耦合电喷雾 用四极离子陷阱质谱仪进行电离。电喷雾 电离已被证明能够产生高带电离子。 来自大的生物分子(MW&GT；104Da)。可以使用四极离子陷阱 为了操纵这些离子以提供分子量和提供 结构信息。这项工作致力于优化接口 在电喷源和质谱仪之间，探索和 优化质量/电荷测量的仪器技术，以及 对高带电粒子的反应性进行基础研究 离子陷阱环境中的生物分子。后一项工作旨在 最大限度地提高灵敏度和结构信息量 获得。这项工作将通过提供 制造精确分子量的相对低成本手段 确定并从广泛的范围获取结构信息 高质量分子。拟议的文书，由于其体积小和 低成本，将使生物医学领域的研究人员更容易获得 社区比目前更大、更昂贵的仪器设备 可用。

The long-term objective of this proposal is to establish the foundation for a relatively small, inexpensive, but very powerful instrument for obtaining structural information from and molecular weights of high mass biomolecules. Specifically, the instrument will couple electrospray ionization with a quadrupole ion trap mass spectrometer. Electrospray ionization has been shown to be capable of producing highly charged ions from large biomolecules (MW > 104 Da). A quadrupole ion trap can be used to manipulate these ions both to provide molecular weights and to provide structural information. This work is devoted to optimizing the interface between the electrospray on source and the mass analyzer, exploring and optimizing instrumental techniques for mass/charge measurement, and performing fundamental research on the reactivities of the highly charged biomolecules in the ion trap environment. The latter work is designed to maximize both sensitivity and the amount of structural information obtained. This work will benefit the biomedical community by providing relatively low-cost means for making accurate molecular weight determinations and for obtaining structural information from a wide range of high mass molecules. The proposed instrument, due to its small size and low cost, would be much more accessible to researchers in the biomedical community than the much larger and more expensive instrumentation presently available.