ANTIFERTILITY EFFECTS OF RESIDUAL AND EXCRETED GOSSYPOL

目前FDA关于肉类日粮中棉酚允许含量的指南 动物和人类是建立在产生一定水平的基础上的 毒性反应。现在人们知道，棉酚作为一种雄性是非常有效的。 抗生育药。然而，棉酚摄取的微妙影响通过 它在动物组织中的残留从未被调查过。 同样，也没有关于潜在环境影响的信息。 从食用棉酚的动物的粪便中排出的棉酚。尽管如此 知识匮乏，棉籽粉(含有高水平的 棉酚)在全世界动物和人类的饮食中被广泛使用 (生产的棉籽粕中有35%是由单胃素消耗的)。我们的 拟议的研究将确定摄取FDA的直接影响 棉酚允许水平(450ppm)对雄性单性生殖的影响 (公猪)食用它以及从其提取的残留棉酚的影响 组织和粪便对实验动物(雄性大鼠)生育力的影响。我们 将为这些提取程序制定方法学。美国食品药品监督管理局。 实验室同意免费提供所需的棉酚 验证我们技术的试点研究，并同意验证 我们提取程序的有效性。几个在体内和体外 对雄性大鼠的生育能力测试以前是我们实验室开发的。 我们的体外测试非常敏感，能够检测到 从组织和组织中提取少量残留棉酚的效果 很快就会产生效果。这结合了个人在 兽医科学、动物科学和肉类科学与技术 进行所有这些活动所需的技能、设施和设备 这项研究的各个方面。这项研究将提供信息来评估 含棉酚残留肉类的安全性、生物活性 排出的棉酚，提供了关于抗生育作用的新信息 并为监管机构提供新的信息。

Present FDA guidelines as to allowable level of gossypol in diets of meat animals and humans were established based upon levels which yielded certain toxic reactions. It is now known that gossypol is highly potent as a male antifertility agent. However, the subtle effects of gossypol ingestion via its residual presence in animal tissue has never been investigated. Likewise, there is no information as to the potential environmental impact of gossypol excreted in the feces of animals consuming it. Despite this paucity of knowledge, cottonseed meal (which contains high level of gossypol) is widely used throughout the world in animal and human diets (35% of cottonseed meal produced is consumed by monogastrics). Our proposed study will determine the direct effects of ingestion of the FDA allowable level of gossypol (450 ppm) on fertility of male monogastrics (boars) consuming it and the effects of residual gossypol extracted from tissue and feces on the fertility of laboratory animals (male rats). We will develop the methodology for these extraction procedures. A U.S.D.A. laboratory has agreed to provide free of charge the gossypol necessary for the pilot studies to validate our technique and also has agreed to verify the validity of our extraction procedures. Several In Vivo and In Vitro tests of fertility in male rats have been previously developed by our lab. Our In Vitro tests are highly sensitive and capable of detecting the effects of small amounts of residual gossypol extracted from tissue and yield a rapid result. This combines the expertise of individuals in veterinary science, animal science and meat science and technology who have the requisite skills, facilities and equipment to conduct all of the many aspects of this study. This study will provide information to assess the safety of meat containing gossypol residues, the biological activity of excreted gossypol, provide new information as to the antifertility effects of gossypol and supply new information for regulatory agencies.