FURTHER ANALYSIS OF HUMAN SPERM FERTILIZING ABILITY

去透明带仓鼠卵子的体外受精最初是 由我们提出作为人类精子受精能力的测试。我们有 随后提供了数据，以确定这种精子的用途 渗透试验以区分可育和潜在的 不育或不能生育的精液样本。许多调查人员都有 验证了这种体外受精程序的能力 评估精液质量。这项测试似乎能够评估 人类精子的获能能力，顶体反应， 和卵子融合。这项建议的目的是研究 目前治疗不孕症的各种治疗方法的有效性 男性。对不育男性治疗方式的评估将 包括使用无透明带卵子穿透试验作为指示 治疗前后生育状况。精索静脉曲张的男性和 不孕症将在手术结扎前和手术后进行检测 精索内静脉。克罗米芬治疗克罗米芬的疗效观察 将通过与男性进行双盲交叉研究来评估 不育症患者。白血球的去除或减少 低生育能力的样本将通过抗生素治疗和 物理移除细胞。白细胞的有效性 作为一种治疗，将通过监测常规的变化来评估移除 无透明带卵子检测系统中的精液参数和渗透率。 第四种治疗方式将包括在培养液中清洗精子。 这可能会增强它们的受精能力，并在宫内 妻子授精以及去透明带仓鼠的授精 鸡蛋。对不育男性的治疗效果进行评估应该会有所帮助 以澄清此类治疗的有效性。使用去透明带卵子检测 我们将消除女性伴侣的混杂变量，从而 让我们的结果不那么模棱两可。

The in vitro fertilization of zona-free hamster eggs was originally proposed by us as a test for human sperm fertilizing ability. We have subsequently presented data to establish the use of such a sperm penetration assay to differentiate between fertile and potentially infertile or subfertile semen samples. Numerous investigators have verified the ability of such an in vitro fertilization procedure to evaluate semen quality. This test appears to be capable of assessing the ability of human spermatozoa to undergo capacitation, acrosome reaction, and ovum fusion. The purpose of this proposal is to study the effectiveness of various treatments currently available for the infertile male. The assessment of treatment modalities for infertile males will involve using the zona-free egg penetration assay as an indicator of fertility status before and after treatment. Men with varicocele and infertility are to be tested before and after surgical ligation of the internal spermatic vein. The effectiveness of clomiphene citrate therapy in men will be evaluated by doing a double blind crossover study with subfertile patients. Removal or reduction of white blood cells in subfertile samples will be accomplished by both antibiotic treatment and physical removal of the cells. The effectiveness of white blood cell removal as a treatment will be evaluated by monitoring changes in routine semen parameters and penetration rates in the zona-free egg test system. The fourth treatment modality will involve washing spermatozoa in media which potentially enhances their fertilizing ability and doing intrauterine insemination in the wife as well as insemination of zona-free hamster eggs. An evaluation of treatment efficacy in subfertile males should help to clarify the validity of such treatments. Using the zona-free egg assay we will eliminate the confounding variable of the female partner and thus make our results less equivocal.