CALCIUM TRANSIENTS IN STRIATED MUSCLE AND OTHER CELLS

钙敏感生物发光蛋白Aequorin和离子选择性 微电极将用于研究细胞内钙离子的调节 在蛙骨骼肌、哺乳动物心肌和 某些其他类型的细胞。将使用高增益图像增强 目的：定位注射马兜铃酸的细胞内的钙离子。关于骨骼肌的研究 将主要旨在了解[Ca++]在体内的调节 静息肌肉，特别强调Na+-Ca++交换，以及 肌浆网和小白蛋白的作用。与中国的关系 静息[Ca++]对静息肌纤维力学性能的影响 探索过了。对心肌的研究将直接针对这种确定 钙离子进入和释放对人体的相对重要性 不同类型心肌的兴奋-收缩偶联 区分钙离子幅度变化的作用 在肌浆网对Ca++的摄取中，以及在 收缩装置在不同压力作用下的敏感性 生理学和药理学正性肌力干预。结果是 应提高对兴奋-收缩耦合的认识 无论是心脏还是骨骼肌，都应该促进更理性的 改善肌肉功能的药物设计。

The calcium-sensitive bioluminescent protein aequorin and ion-selective microelectrodes will e used to study the regulation of intracellular Ca++ concentration in frog skeletal muscle, mammalian cardiac muscle, and certain other types of cells. High-gain image intensification will be used to localize Ca++ in aequorin-injected cells. Studies in skeletal muscle will be aimed primarily at understanding the regulation of [Ca++] in resting muscle, with particular emphasis on Na+-Ca++ exchange, and the roles of the sarcoplasmic reticulum and parvalbumin. The relation of resting [Ca++] to mechanical properties of resting muscle fibers will be explored. Studies in cardiac muscle will be directed at the determination of the relative importance of Ca++ entry and Ca++ release for excitation-contraction coupling in different types of heart muscle, and at differentiating the roles of changes in the amplitude of the Ca++ transient, in Ca++ uptake by the sarcoplasmic reticulum, and in the sensitivity of the contractile apparatus in the action of various physiological and pharmacological inotropic interventions. The results should increase our understanding of excitation-contraction coupling in both heart and skeletal muscle, and should facilitate a more rational design of drugs for the improvement of muscle function.