SECRETION AND ACTION OF DECIDUAL LUTEOTROPIN

这项建议的总体目标是获得进一步的洞察力 蜕膜促黄体素的分泌和作用，一种催乳素- 就像孕妇蜕膜组织分泌的激素一样 假孕大鼠。第一个目标将是建立一个直接的 蜕膜促黄体激素对黄体和卵泡的作用 类固醇合成。在这项研究中，卵泡和黄体细胞 要么用从蜕膜细胞流出的培养液 灌流，或将与高度纯化的激素孵化。 利用体外和体内的方法，我们将研究 蜕膜促黄体素局部作用于蜕膜的可能性 邻近的滋养层细胞通过以下方式控制糖原的产生 蜕膜和滋养层细胞的类固醇合成。第三 为确定蜕膜的个体发育而设计的特定目标 促黄体生成素贯穿蜕膜的整个生命周期 组织。到目前为止，除了女人和老鼠，它还不是 已知类催乳素物质的生产是否是 蜕膜组织的激素功能。这件事的最终目的是 研究将确定在其他物种中 蜕膜化伴随着植入，催乳素样物 激素是由蜕膜组织产生的。这将会 建立蜕膜作为内分泌腺的物种 血绒毛胎盘。我们还将调查这一可能性 在其中一些物种中，蜕膜催乳素具有 促黄体活性。

The overall objective of this proposal is to gain further insight into the secretion and action of decidual luteotropin, a prolactin- like hormone secreted by the decidual tissue of pregnant and pseudopregnant rats. The first aim will be to establish a direct action of decidual luteotropin on luteal and follicular steroidogenesis. For this investigation, follicles and luteal cells will be either perifused with medium flowing from a decidual cell perifusion, or will be incubated with highly purified hormone. Using in vitro and in vivo approaches, we will investigate the possibility that decidual luteotropin acts locally on decidual and neighboring trophoblastic cells to control glycogen production by the decidua, and steroidogenesis by trophoblastic cells. The third specific aim is designed to determine the ontogeny of decidual luteotropin production throughout the life span of the decidual tissue. To date, with the exception of women and rats, it is not known whether the production of a PRL-like substance is a hormonal function of the decidual tissue. The last aim of this study will be to determine whether in other species in which decidualization accompanies implantation, prolactin-like hormones are produced by the decidual tissue. This would establish the decidua as an endocrine gland in species with hemochorial placentation. We will also investigate the possibility that in some of these species, decidual prolactin possesses luteotropic activity.