GROWTH AND PUBERTY

营养不足或过度运动对青春期的抑制是 有相当的临床意义。 这一现象的背后是一个困难的 生理学问题：“青春期是如何代谢调节的， 即使是营养良好的女性， 不做大量运动吗“大多数雌性哺乳动物都会经历青春期 只有当他们达到特定的体型或组成时。 这 意味着营养或能量之间的某种代谢联系 加工和促性腺激素分泌。 我们几乎不知道 这种联系。 这项提案的目的是探讨食物与 女性摄入、运动、代谢、生长、内分泌与青春期 大鼠 我发明了一种喂养模式， 控制LH的脉冲式分泌。 我打算用这个范例 以确定食物的哪个维度（卡路里、蛋白质、特定 氨基酸等）实际上控制着GnRH脉冲发生器 此外，我计划了解是否有一个单独的， 参与GnRH控制的特异性、血源性、代谢信号 分泌物 我的内分泌问题是广泛的：它们包括在 体内和体外方法，以及GH，TSH，FSH和 Prl，以及LH。 作为比较方法，我打算同样详细地研究 生理途径参与抑制青春期的过度 锻炼的 我发明了一种笼子系统，幼鼠必须使用 轮子来获取食物。 初步研究表明， 通过增加能量消耗来抑制青春期， 同时允许正常的能量和营养摄入。 最终我 我想开发两个大的数据集使用喂养和锻炼 范例 这两个数据集结合在一起， 关于青春期是由卡路里和营养素调节的假说， 正常发育的女性

The inhibition of puberty by undernourishment or excessive exercise is of considerable clinical interest. Underlying this phenomenon is a difficult physiological question: "how is puberty regulated metabolically and endocrinologically by body growth, even in the well-nourished female who does not exercise extensively?" Most female mammals experience puberty only when they have achieved a particular body size or composition. This implies a metabolic linkage of some kind between nutrient or energy processing and gonadotropin secretion. We know virtually nothing about this linkage. The object of this proposal is to explore the relationship between food intake, exercise, metabolism, growth, endocrines and puberty in female rats. I have developed a feeding paradigm that allows a great degree of control over the pulsatile secretion of LH. I intend to use this paradigm to determine which dimension of food (calories vs. protein vs. specific amino acids, etc.) actually controls the GnRH pulse generator. Furthermore, I plan to find out whether or not there is a separate and specific, bloodborne, metabolic signal involved in the control of GnRH secretion. My endocrine concerns here are broad: they include both in vivo and in vitro approaches, and the roles played by GH, TSH, FSH, and Prl, as well as LH. As a comparative approach, I intend to study in the same detail the physiological pathways involved in the inhibition of puberty by excessive exercise. I have developed a caging system in which young rats must use a running wheel to obtain their food. Pilot studies suggest that I can inhibit puberty with this system by increasing energy expenditure, even while allowing otherwise normal energy and nutrient intake. Ultimately I want to develop two large sets of data using the feeding and exercise paradigms. Together these two data sets should yield new testable hypotheses about the way puberty is regulated by calories and nutrients in normally growing females.