GROWTH AND PUBERTY

营养不良或过度运动对青春期的抑制是 相当大的临床兴趣。在这种现象的背后是一个困难的 生理问题：青春期是如何代谢和调节的？ 在内分泌方面，通过身体生长，即使在营养良好的雌性 大多数雌性哺乳动物都会经历青春期？ 只有当他们达到了特定的身体尺寸或组成时。这 暗示营养或能量之间存在某种新陈代谢联系 加工和促性腺激素分泌。我们对此几乎一无所知 这种联系。 这项提议的目的是探索食物和食物之间的关系 女性的摄入量、运动、代谢、生长、内分泌和青春期 老鼠。我已经开发了一种喂养模式，可以在很大程度上 控制促黄体生成素的脉动性分泌。我打算使用这个范例 确定食物的哪个维度(卡路里、蛋白质和特异值 氨基酸等)实际上控制着GnRH脉冲发生器。 此外，我计划调查是否有单独的和 与促性腺激素释放激素控制有关的特异性、血源性代谢信号 分泌物。我在这里对内分泌的担忧是广泛的：它们都包括 体内和体外途径，以及GH、TSH、FSH和 催乳素(PRL)和黄体生成素(LH)。 作为一种比较的方法，我打算同样详细地研究 过量抑制青春期的生理途径 锻炼身体。我已经开发了一种笼子系统，在这种系统中，幼鼠必须使用 转动轮子来获取它们的食物。初步研究表明，我可以 通过增加能量消耗来抑制青春期，甚至 同时允许正常的能量和营养摄入。最终我 我想使用喂养和锻炼来开发两个大型数据集 范例。这两个数据集加在一起应该会产生新的可测试性 关于青春期受卡路里和营养调节方式的假说 正常生长的雌性。

The inhibition of puberty by undernourishment or excessive exercise is of considerable clinical interest. Underlying this phenomenon is a difficult physiological question: "how is puberty regulated metabolically and endocrinologically by body growth, even in the well-nourished female who does not exercise extensively?" Most female mammals experience puberty only when they have achieved a particular body size or composition. This implies a metabolic linkage of some kind between nutrient or energy processing and gonadotropin secretion. We know virtually nothing about this linkage. The object of this proposal is to explore the relationship between food intake, exercise, metabolism, growth, endocrines and puberty in female rats. I have developed a feeding paradigm that allows a great degree of control over the pulsatile secretion of LH. I intend to use this paradigm to determine which dimension of food (calories vs. protein vs. specific amino acids, etc.) actually controls the GnRH pulse generator. Furthermore, I plan to find out whether or not there is a separate and specific, bloodborne, metabolic signal involved in the control of GnRH secretion. My endocrine concerns here are broad: they include both in vivo and in vitro approaches, and the roles played by GH, TSH, FSH, and Prl, as well as LH. As a comparative approach, I intend to study in the same detail the physiological pathways involved in the inhibition of puberty by excessive exercise. I have developed a caging system in which young rats must use a running wheel to obtain their food. Pilot studies suggest that I can inhibit puberty with this system by increasing energy expenditure, even while allowing otherwise normal energy and nutrient intake. Ultimately I want to develop two large sets of data using the feeding and exercise paradigms. Together these two data sets should yield new testable hypotheses about the way puberty is regulated by calories and nutrients in normally growing females.