HUMAN PLACENTAL ATRIAL NATRIURETIC FACTOR RECEPTORS

心房利钠因子（ANF），最近发现的多肽 激素，是一种最有效的天然钠尿肽 和利尿剂。 与其他激素类似，其细胞 作用可能是由ANF-受体相互作用介导的。 的 本研究的目的是生物化学地表征， 从物理化学和免疫学角度来看， 人胎盘 我们还将描述ANF诱导的 内源性底物的磷酸化， 最近在胎盘膜中观察到， 与受体的关系。 我们将纯化受体， 亲和色谱法;建立最佳条件， 激素与其结合的结构要求 受体;测定其理化结构;研究 多结合位点可能性和制备抗体 对抗受体蛋白。 纯化受体的可用性， 抗受体抗体将使我们能够实现我们的长期 确定组织、细胞和亚细胞分布的目标 心房利钠因子受体;阐明其生物合成 在人体组织中;检测其相关的结构差异 具有生理或病理生理条件，例如 妊娠合并高血压，也许 在治疗上干预其致病过程， 贡献。

Atrial natriuretic factor (ANF), a recently discovered polypeptide hormone, is one of the most potent naturally occurring natriuretic and diuretic agents. By analogy to other hormones, its cellular effects are probably mediated by ANF-receptor interactions. The aims of this research are to characterize biochemically, physicochemically, and immunologically ANF receptor from human placenta. We will also characterize the ANF-induced phosphorylation of an endogenous substrate which we have recently observed in placental membranes and establish its relationship to the receptor. We will purify the receptor by affinity chromatography; establish optimal conditions and structural requirements for binding of the hormone to its receptors; determine its physicochemical structure; investigate the possibility of multiple binding site & prepare antibodies against receptor protein. Availability of purified receptor and antireceptor antibodies will enable us to fulfill our long-term goals of determining tissue, cellular, and subcellular distribution of atrial natriuretic factor receptors; elucidating its biosynthesis in human tissues; detecting structural differences in it associated with physiological or pathophysiological conditions such as pregnancies complicated with hypertension, and perhaps intervening therapeutically in the pathogenic process to which it contributes.