EARLY ASPECTS OF FOLLICULAR DEVELOPMENT

这项研究的总体目标是了解 指导大鼠卵巢卵泡的组装和早期生长。这个 本提案中描述的研究涉及以下基本问题 关于卵泡发生的第一步的问题： 1)内膜细胞在发育过程中与颗粒细胞相关，还是与 结缔组织细胞？ 2)内膜前体细胞是否与颗粒一起搁置 原始卵泡组装过程中的祖细胞还是它们会出现 在生命中，每个原始卵泡都开始 成长？ 3)是什么刺激了休眠的原始毛囊开始生长？ A)这一过程是否处于监管反馈控制之下？ B)是地方一级、卵巢内一级或 全球层面？ C)转化生长因子α和转化生长因子β是否在唤醒休眠卵泡中起作用？ 4)卵泡基底膜如何扩张以适应 在卵泡生长过程中卵泡体积的巨大增加？ 5)哪些细胞负责沉积和维持基底 基底膜的板层成分？ 6)卵母细胞是否诱导周围的体细胞沉积基底 卵巢组织发生过程中的膜？ 这些问题将通过活体研究来解决，包括 逆转录病毒介导的基因转移以追踪细胞谱系，同种异体移植 胚胎和婴儿卵巢到成年宿主的肾被膜 区分环境监管和内在监管，长期- 持续输注[~3H]胸腺嘧啶核苷以标记缓慢增殖的细胞，以及 基底膜成分的免疫组织化学检测 光镜和电子显微镜水平。此外，还合成了该化合物 基底膜将在体外进行研究。

The overall goal of this research is to understand the mechanisms that direct the assembly and early growth of the ovarian follicle in rats. The studies described in this proposal address the following fundamental questions concerning the first steps in folliculogenesis: 1) Are theca interna cells developmentally related to granulosa cells or to connective tissue cells? 2) Are theca interna progenitor cells set aside along with granulosa progenitor cell during assembly of primordial follicles or do they arise continuously throughout life after each primordial follicle has begun to grow? 3) What stimulates dormant primordial follicles to begin growth? a) Is this process under regulatory feedback control? b) Is the locus of control at the local, intraovarian level or at the global level? c) Do TGFalpha and TGF beta play a role in arousing dormant follicles? 4) How does the follicular basement membrane expand to accommodate the enormous increase in follicular volume during follicular growth? 5) Which cells are responsible for depositing and maintaining the basal lamina component of the basement membrane? 6) Does the oocyte induce surrounding somatic cells to deposit a basement membrane during ovarian histogenesis? These questions will be addressed with in vivo studies involving retrovirus-mediated gene transfer to trace cell lineages, isografting of embryonic and infant ovaries to the kidney capsules of adult hosts to distinguish between environmental vs. intrinsic regulation, long term- continuous [3H] thymidine infusion to label slowly proliferating cells, and immunohistochemical detection of basement membrane components at both the light and electron microscopic levels. In addition, synthesis of the basement membrane will be studied in vitro.