EOSINOPHIL MAJOR BASIC PROTEIN IN HUMAN PREGNANCY

先前的研究表明，嗜酸性粒细胞颗粒含有一种 被称为主要碱性的强阳离子蛋白质分子 蛋白质(GMBP)。豚鼠MBP占50%以上 颗粒蛋白和细胞总蛋白的25%。GMBP为 定位于颗粒的核心，以晶体的形式存在。 在人类中也发现了gMBP；豚鼠和 人gMBP对蠕虫和哺乳动物细胞有很强的毒性； 而gMBP已被认为是一种病理性的介体。 对哮喘和其他炎症性疾病的损害。 在研究gMBP在过敏性疾病中的作用时， 我们发现人类体内gMBP水平升高 怀孕了。MBP水平在受孕后不久上升，持平于 大约20周，并在临产前不久出现晚起。这个 妊娠相关主要碱性蛋白(PMBP)的行为 在免疫化学性质上与gMBP相同，但最初 研究表明，分子量不同。在最近 研究发现，pMBP水平的后期上升是 与人类劳动有关的。 在这个提议中，我们概述了研究，首先，从 胎盘的数量足以进行化学分析。第二, 我们将测试纯化的pMBP是否与gMBP相同。这些 实验预测了我们提纯pMBP的能力以及其他 关于gMBP的先前物理化学信息。第三，我们 将在人类怀孕期间测量血清pMBP水平 在100名正常孕妇和30名对照组中 未怀孕的女性。第四，我们将测量血清pMBP 在各种病理条件下的水平与 怀孕，包括对以下假设的测试： 36周前的PMBP预示早产。我们还将测试 PMBP在延长的管理中是否有用 妊娠和是否复发患者的一个亚组 流产患者的血浆pMBP水平可能存在异常。 总体而言，这些实验将决定自发的 可以通过pMBP的后期上升来预测劳动力，他们提供了 希望获得有关该功能的新信息 人类妊娠中相对被忽视的滋养层细胞称为 X细胞。

Prior studies have shown that the eosinophil granule contains an intensely cationic proteinaceous molecule termed the major basic protein (gMBP). Guinea pig MBP accounts for over 50% of granule proteins and 25% of the total cellular protein. gMBP is localized to the core of the granule and exists there as a crystal. gMBP has also been identified in the human; both guinea pig and human gMBP are potent toxins to helminths and mammalian cells; and gMBP has been proposed as a mediator of pathological damage in bronchial asthma and other inflammatory diseases. During studies of the role of gMBP in hypersensitivity diseases, we found that elevated levels of gMBP are present in human pregnancy. MBP levels rise shortly after conception, plateau at about 20 weeks and show a late rise shortly before labor. The pregnancy-associated major basic protein (pMBP) behaves identically to gMBP in its immunochemical properties, but initial studies showed a difference in molecular weight. In recent studies, we have found that a late rise in pMBP levels is associated with human labor. In this proposal, we outline studies, first, to purify pMBP from placenta in quantities sufficient for chemical analyses. Second, we will test whether purified pMBP is identical to gMBP. These experiments are predicted on our ability to purify pMBP and on prior physicochemical information concerning gMBP. Third, we will measure levels of serum pMBP throughout human pregnancy in 100 normal pregnant women and in a control group of 30 nonpregnant women. And fourth, we will measure serum pMBP levels in various pathological conditions associated with pregnancy, including tests of the hypothesis that elevations of pMBP before 36 weeks predict premature labor. We also will test whether pMBP is useful in the management of prolonged pregnancy and whether a subset of patients with recurrent abortions may have abnormalities in the plasma levels of pMBP. Overall, these experiments will determine whether spontaneous labor can be predicted by late rises in pMBP and they offer the hope of obtaining new information concerning the function in human pregnancy of the relatively neglected trophoblast known as the X cell.