HIGH RESOLUTION GENETIC MAPPING: STRATEGY-BASED APPROACH

这项研究的总体目标是开发一种高级资源 分辨率作图，最初用于17号染色体和X染色体上的标记；以及 随后，针对其他染色体。该资源将由 鉴定60CEPH中交叉确定的减数分裂片段 家人。减数分裂作图面板将大大提高效率 和遗传图谱的可靠性。CEPH的潜在拆分能力 常染色体约0.01 cM，X染色体约0.02 cM。 为了最有效地开发地图面板资源，将 需要修改和扩展现有的计算机程序，这些程序 目前用于CEPH标记数据的单倍型和质量控制。 目前17号染色体和X号染色体的标记密度足以 使用这些简单的算法毫不含糊地识别几乎所有的交叉。 每个分叉的位置将通过重复键入 最近的侧翼标记。将对更多的减数分裂进行筛选 紧密连锁的基因座之间的重组子不能被其他 意思是。其他标记的选择性分型将在定向的 努力弥合每个跨界车周围的差距。这一战略将 提高分辨率，同时完善 新的记号笔。用于将遗传数据转换为 物理映射表示将被开发为一种简单的方法 结合和比较遗传作图和物理作图的结果 学习。

The overall objective of this study is to develop a resource for high resolution mapping, initially for markers on chromosomes 17 and X; and subsequently, for other chromosomes. The resource will be developed by identifying meiotic fragments that are defined by crossovers in 60 CEPH families. The meiotic mapping panel will greatly improve the efficiency and reliability of genetic mapping. The potential resolution of the CEPH resource is about 0.01 cM for autosomes and 0.02 cM for chromosome X. In order to develop the mapping panel resource most efficiently, it will be necessary to modify and extend existing computer programs that are currently used for haplotyping and quality control of CEPH marker data. The current density of markers for chromosomes 17 and X is sufficient to identify nearly all crossovers unambiguously using these simple algorithms. The position of each crossover will be validated by duplicate typing of the nearest flanking markers. Additional meioses will be screened for recombinants between tightly linked loci that cannot be ordered by other means. Selective typing of additional markers will be done in a directed effort to close the gap surrounding each crossover. This strategy will improve resolution and, at the same time, refine the localization of the new marker. Computer algorithms for translating genetic data into a physical mapping representation will be developed as a simple means of combining and comparing the results of genetic and physical mapping studies.