EARLY ASPECTS OF FOLLICULAR DEVELOPMENT

本研究的总体目标是了解 指导大鼠卵泡的组装和早期生长。 的 本提案中所述的研究涉及以下基本问题 关于卵泡形成的第一步的问题： 1)内膜细胞与颗粒细胞的发育相关吗？ 结缔组织细胞 2)内膜祖细胞是否与颗粒层一起沿着 在原始卵泡的组装过程中祖细胞，或者它们是否产生 在每个原始卵泡开始发育后， 成长？ 3)是什么刺激休眠的原始卵泡开始生长？ a）该过程是否处于监管反馈控制之下？ B）控制点是在局部、卵巢内水平还是在 全球层面？ c）TGF α和TGF β在唤醒休眠卵泡中发挥作用吗？ 4)卵泡基底膜是如何扩张以容纳 在卵泡生长过程中卵泡体积的巨大增加？ 5)哪些细胞负责沉积和维持基底细胞 基底膜的薄层成分？ 6)卵母细胞是否诱导周围的体细胞存款基底 膜在卵巢组织发生中的作用？ 这些问题将通过体内研究来解决， 逆转录病毒介导的基因转移到微量细胞系，同种移植， 胚胎和婴儿的卵巢到成年宿主的肾囊， 区分环境与内在调节，长期- 连续输注[3H]胸苷以标记缓慢增殖的细胞，和 免疫组化检测基底膜成分在两个 光学和电子显微镜水平。 此外，合成的 将在体外研究基底膜。

The overall goal of this research is to understand the mechanisms that direct the assembly and early growth of the ovarian follicle in rats. The studies described in this proposal address the following fundamental questions concerning the first steps in folliculogenesis: 1) Are theca interna cells developmentally related to granulosa cells or to connective tissue cells? 2) Are theca interna progenitor cells set aside along with granulosa progenitor cell during assembly of primordial follicles or do they arise continuously throughout life after each primordial follicle has begun to grow? 3) What stimulates dormant primordial follicles to begin growth? a) Is this process under regulatory feedback control? b) Is the locus of control at the local, intraovarian level or at the global level? c) Do TGFalpha and TGF beta play a role in arousing dormant follicles? 4) How does the follicular basement membrane expand to accommodate the enormous increase in follicular volume during follicular growth? 5) Which cells are responsible for depositing and maintaining the basal lamina component of the basement membrane? 6) Does the oocyte induce surrounding somatic cells to deposit a basement membrane during ovarian histogenesis? These questions will be addressed with in vivo studies involving retrovirus-mediated gene transfer to trace cell lineages, isografting of embryonic and infant ovaries to the kidney capsules of adult hosts to distinguish between environmental vs. intrinsic regulation, long term- continuous [3H] thymidine infusion to label slowly proliferating cells, and immunohistochemical detection of basement membrane components at both the light and electron microscopic levels. In addition, synthesis of the basement membrane will be studied in vitro.