NEW APPROACHES TO DNA MAPPING--ELECTRONIC IMAGING

限制性内切酶片段的凝胶分离与分析 凝胶电泳法和Southern blotting法对基因图谱的定位至关重要 染色体DNA。然而，目前获得的信息 从凝胶和斑点中分离出来受到非线性响应的严重限制， 动态范围小、灵敏度低、处理繁琐 摄影方法中固有的用于记录 荧光团染色或放射性核素标记的DNA的分布。 该项目将利用电子成像领域的最新进展。 和计算机技术，使准确和快速成为可能 荧光染料质量分布的定量研究 凝胶上的限制性片段和放射性核素标记的探针 南方印迹。用于存储和分析的计算机程序 凝胶和斑点的图像将被显影以增加 值得注意的是，所获得的信息的质量和数量 同时减少数据收集和 正在处理。

Separation and analysis of restriction fragments by gel electrophoresis and Southern blotting are vital to the mapping of chromosomal DNA. At present, however, the information obtained from gels and blots is severely limited by the nonlinear response, limited dynamic range, low sensitivity and cumbersome processing inherent in the photographic methods used to record the distribution of fluorophore-stained or radionuclide-labeled DNA. This project will exploit recent advances in electronic imaging and computer technology that make possible accurate and rapid quantitation of the mass distribution of fluorophore-stained restriction fragments on gels and radionuclide-labeled probes on Southern blots. Computer programs for the storage and analysis of images of gels and blots will be developed to increase significantly the quality and quantity of information obtained while reducing the time required for data collection and processing.