NEW APPROACHES TO DNA MAPPING--ELECTRONIC IMAGING

酶切片段的凝胶分离与分析 电泳和Southern印迹是至关重要的映射 染色体DNA 然而，目前获得的信息 从凝胶和印迹中分离的方法受到非线性响应的严重限制， 有限的动态范围、低灵敏度和繁琐的处理 固有的摄影方法用来记录 荧光团染色或放射性核素标记的DNA的分布。 这个项目将利用电子成像的最新进展 和计算机技术，使准确和快速 定量荧光团染色的 凝胶上的限制性片段和放射性核素标记的探针 Southern blots 存储和分析的计算机程序 将开发凝胶和印迹图像， 获得的信息的质量和数量 同时减少数据收集所需的时间， 处理.

Separation and analysis of restriction fragments by gel electrophoresis and Southern blotting are vital to the mapping of chromosomal DNA. At present, however, the information obtained from gels and blots is severely limited by the nonlinear response, limited dynamic range, low sensitivity and cumbersome processing inherent in the photographic methods used to record the distribution of fluorophore-stained or radionuclide-labeled DNA. This project will exploit recent advances in electronic imaging and computer technology that make possible accurate and rapid quantitation of the mass distribution of fluorophore-stained restriction fragments on gels and radionuclide-labeled probes on Southern blots. Computer programs for the storage and analysis of images of gels and blots will be developed to increase significantly the quality and quantity of information obtained while reducing the time required for data collection and processing.