FRAMEWORK LINKAGE MAPS FOR CHROMOSOMES 2 6 7 8 12 & 14

我们建议构建人类框架遗传连锁图谱 染色体2、6、7、8、12和14。这些图谱将包括 最低杂合度为70%的DNA序列多态和标记 间隔一般不超过10%的复合。略高一点 在提供标记信息的情况下，将允许重组间隔 远远高于70%。初始地图(男性、女性和性别 Average)将从CEPH参考家系基因型中构建，使用 连锁分析程序包CRI-MAP和这些将作为 确定需要开发探测器的区域的依据。CA重复 多态将是待识别的新标记的主要类型 特色化的。半自动图像分析程序将开发为 CA基因多态的基因分析辅助工具。初始设置 提出了每条染色体的候选索引标记，以供研究。一个 检验双等位基因标记可行性的新方法 自动化基因分型分析被建议用于14号染色体的定位 与更传统的方法相结合。

We propose to construct framework genetic linkage maps for human chromosomes 2, 6, 7, 8, 12, and 14. The maps will consist of markers for DNA sequence polymorphism with minimum heterozygosities of 70% and marker spacing, in general, of no more than 1 0% recombination. Slightly higher recombination intervals will be allowed provided marker informativeness is considerably higher than 70%. Initial maps (male, female, and sex average) will be constructed from CEPH reference pedigree genotypes using the linkage analysis program package CRI-MAP and these will serve as the basis for determining regions requiring probe development. CA repeat polymorphisms will be the main type of new markers to be identified and characterized. Semiautomated image analysis programs will be developed as an aid to genotype analysis for CA polymorphisms. Initial sets of candidate index markers from each chromosome are proposed for study. A novel approach to test the feasibility of bi-allelic markers and automated genotyping assays is proposed for chromosome 14 mapping in conjunction with more traditional approaches.