FETAL AND PLACENTAL METABOLISM OF THREONINE AND ALANINE

这些研究将检查胎儿和胎盘的新陈代谢 氨基酸：1)一种必需的氨基酸，苏氨酸和丙氨酸。两者都有 是潜在的葡萄糖前体。这些研究将比较它们的比率 对胎儿和胎盘的供应 要求。这些研究将长期进行。 留置尿管怀孕绵羊，在稳态无应激状态下进行研究 条件。将使用多种示踪方法来研究它们的 胎盘、胎肝和胎肝在胎儿体内的代谢 新陈代谢是可以分离的。氧化率、等离子体处理率 肝脏对这些氨基酸的净摄取和新陈代谢 在正常条件下和在两个不同的模型中进行研究 胎儿宫内发育迟缓，与血糖降低有关 对胎儿的分娩和葡萄糖生成以及另一例正常血糖 送货。将测量进出的净示踪剂和示踪剂通量 胎儿、胎肝和子宫胎盘。碳标签 苏氨酸和丙氨酸代谢的潜在产品也将是 测定，特别是其他氨基酸，葡萄糖和乳酸。

These studies will examine the fetal and placental metabolism of two amino acids: 1) an essential amino acid, threonine and 2) alanine. Both are potential glucose precursors. The studies will compare their rates of supply to the fetus and placenta with the fetal and placental requirements. The studies will be carried out in chronically catheterized pregnant sheep, studied under steady state unstressed conditions. Multiple tracer methodology will be used to study their metabolism in fetuses in which placental, fetal and fetal hepatic metabolism can be separated. The oxidation rate, plasma disposal rates and net hepatic uptake and metabolism of these amino acids will be studied under normal conditions and in two different models associated with intrauterine growth retardation, one associated with reduced glucose delivery to the fetus and glucogenesis and the other with normal glucose delivery. Net tracer and tracee fluxes will be measured into and out of the fetus, the fetal liver and the uteroplacenta. Carbon labelling of potential products of threonine and alanine metabolism will also be determined, particularly for other amino acids, glucose and lactate.