CYCLIC NUCLEOTIDE METABOLISM IN LUNG & VASCULAR TISSUES

环核苷酸在肺和某些组织中的形成和作用的调节 它的细胞类型，包括气管平滑肌，将被检查。这个 环磷酸鸟苷和环磷酸腺苷在气管平滑肌中的蓄积 含有各种激素、神经激素、亚硝基化合物等材料 将与肌肉运动和内源性蛋白相关 磷酸化。外源和内源蛋白质底物用于环化 肺和气管平滑肌中的核苷酸依赖蛋白激酶 将会被检查。肺中的鸟苷环化酶将被表征， 纯化并用于获得抗体，这些抗体将用于开发 酶的放射免疫分析及免疫组织化学定位 肺中的酶。因此，实验将在肺和一些 它的细胞类型决定了哪些制剂调节蓄积和 环核苷酸的作用试图将某些功能归因于 肺组织中cAMP和cGMP的含量。

The regulation of cyclic nucleotide formation and action in lung and some of its cell types including tracheal smooth muscle will be examined. The accumulation of cyclic GMP and cyclic AMP in tracheal smooth muscle induced with various hormones, neurohormones, nitroso compounds and other materials will be correlated with muscle motility and endogenous protein phosphorylation. Exogenous and endogenous protein substrates for cyclic nucleotide dependent protein kinases in lung and tracheal smooth muscle will be examined. Guanylate cyclase in lung will be characterized, purified and used to obtain antibodies that will be used to develop a radioimmunoassay for the enzyme and immunohistochemical localization of the enzyme in lung. Thus, experiments will be performed with lung and some of its cell types to determine which agents regulate the accumulation and action of cyclic nucleotides in an attempt to ascribe some functions to cyclic AMP and cyclic GMP in lung.