MICROCIRCULATORY FLOW OF SICKLE BLOOD

目标是帮助确定地点、机制和 镰刀病致微循环血管闭塞的动力学研究 红细胞，以便更好地了解导致 对镰状细胞疾病患者的危象，并将选择 在更合理的基础上采取潜在的治疗措施。 红细胞和正常细胞混合物的血液和其他悬浮液 硬化、变形的红细胞将通过真实比例模型被泵送 微血管，有或没有同时向或向其传递氧气 鲜血。泥沙流动的微观观察与测量 重要参数(如流动中的局部血氧饱和度水平 血液)将允许表征当血管闭塞时的情况 发生。通过不同的组成和特征属性 红细胞和细胞悬浮液，以及流动条件和氧气 饱和程度，红细胞变形性特征的作用， 细胞-血管壁黏附和血红蛋白凝胶动力学 微血管闭塞将被确定。 实验过程中氧传输过程的数学模型 将使用流动系统来定义相关的边界条件 并对实验结果进行分析。数学上的 建模将包括对氧运输、氧-血红蛋白的研究 反应和脱氧血红蛋白凝胶化过程 流动的红细胞；模型将用实验数据进行测试。

The objectives are to help to ascertain the locations, mechanisms and kinetics of vasocclusion of the microcirculation by sickle disease erythrocytes, so as to permit a better understanding of the events leading to crises in patients with sickle cell disease, and to put selection of potential therapeutic measures on a more rational basis. Bloods and other suspensions of erythrocytes and mixtures of normal cells and hardened, deformed erythrocytes will be pumped through real-scale model microvasculatures, with or without simultaneous oxygen transfer from or to the blood. Microscopic observation of the flow and measurement of important parameters (such as local oxygen saturation levels in the flowing blood) will permit characterization of conditions when vasocclusion occurs. By varying compositional and characteristic properties of the erythrocytes and cell suspensions, as well as flow conditions and oxygen saturation levels, the roles of erythrocyte deformability characteristics, cell-vessel wall adherence and hemoglobin gelation kinetics in microvascular vasocclusion will be ascertained. Mathematical models of the oxygen transport processes in the experimental flow system will be used to define boundary conditions for pertinent experiments and to analyze the experimental results. The mathematical modelling will include studies of the oxygen transport, oxygen-hemoglobin reaction, and deoxyhemoglobin gelation processes within stationary and flowing erythrocytes; models will be tested with experimental data.