MICROCIRCULATORY FLOW OF SICKLE BLOOD

其目的是帮助确定地点、机制和 镰状病微循环血管阻塞的动力学 红细胞，以便更好地了解导致 镰状细胞病患者的危机，并将选择 在更合理的基础上采取潜在的治疗措施。 血液和其他红细胞悬浮液以及正常细胞的混合物 硬化变形的红细胞将通过真实比例的模型 微血管，有或没有同时从或向 血 流动的显微观察和 重要的参数（例如流动中的局部氧饱和度水平 血液）将允许表征血管阻塞时的状况 发生。 通过改变组合物的组成和特征性质， 红细胞和细胞悬浮液，以及流动条件和氧气 饱和水平，红细胞变形能力特征的作用， 细胞-血管壁粘附和血红蛋白胶凝动力学 将确定微血管闭塞。 实验中氧输运过程的数学模型 流动系统将用于定义相关的边界条件 并对实验结果进行分析。 数学 建模将包括氧气运输、氧血红蛋白 反应，和脱氧血红蛋白凝胶化过程中的固定和 流动的红细胞;将用实验数据测试模型。

The objectives are to help to ascertain the locations, mechanisms and kinetics of vasocclusion of the microcirculation by sickle disease erythrocytes, so as to permit a better understanding of the events leading to crises in patients with sickle cell disease, and to put selection of potential therapeutic measures on a more rational basis. Bloods and other suspensions of erythrocytes and mixtures of normal cells and hardened, deformed erythrocytes will be pumped through real-scale model microvasculatures, with or without simultaneous oxygen transfer from or to the blood. Microscopic observation of the flow and measurement of important parameters (such as local oxygen saturation levels in the flowing blood) will permit characterization of conditions when vasocclusion occurs. By varying compositional and characteristic properties of the erythrocytes and cell suspensions, as well as flow conditions and oxygen saturation levels, the roles of erythrocyte deformability characteristics, cell-vessel wall adherence and hemoglobin gelation kinetics in microvascular vasocclusion will be ascertained. Mathematical models of the oxygen transport processes in the experimental flow system will be used to define boundary conditions for pertinent experiments and to analyze the experimental results. The mathematical modelling will include studies of the oxygen transport, oxygen-hemoglobin reaction, and deoxyhemoglobin gelation processes within stationary and flowing erythrocytes; models will be tested with experimental data.