PLATELET ACTIVATION & POLYMER SURFACE THROMBOGENICITY

目的是了解血小板黏附和活化是如何受到影响的。 通过聚合物的表面性质，这些聚合物被选择用于其 在生物医学应用中的潜力，并将这些反应与 它们对血栓形成的影响。 纯化的人血小板在聚合物表面的激活，这具有 以前用X射线光电子能谱、接触式 角度和其他技术；将通过扫描电子来研究 显微镜，高压电子显微镜，在活着的状态下，由 视频增强型差分干涉对比光显微镜。 细胞骨架重组在变形、时间和时间上的差异 血小板表面变形率、外观及运动能力 将对每个表面的受体和颗粒分泌进行分析。 胶体金标记的单抗和特异性配体 将被用作探针来跟踪受体运动，由光和 电子显微镜。然后将分析形状变化的差异 使用药理学药物改变激活、受体表达和 细胞骨架重组。然后，这些观察结果将与 聚合物的物理化学性质。此外，还有三个参数 影响血栓形成的血小板功能将在体外分析 不同的聚合物。这些是；血小板致密颗粒分泌 光致发光三磷酸腺苷测定、受体免疫金珠研究 表达，以及由血小板激动剂诱导的激活程度。 通过迭代过程，具有其他表面性质的聚合物将 接受检查。最终，聚合物表面性质的测定 在调节血小板黏附和激活过程中起重要作用的是 想要。 对血小板对不同类型聚合物反应的理解 表面性质可以为开发具有最低限度的聚合物提供基础 当放置在循环中时会产生不良影响。了解以下内容 血小板改变其对表面反应的机制，如所研究的 通过药理手段，还可以改善抗凝血剂 当人造材料被放入血液循环时的治疗。在……里面 此外，受体的外观和运动性的观察，以及颗粒 分泌将有助于更好地了解血小板在 血栓形成的调控及体外表面检测方法的改进 血栓形成能力。

The aim is to understand how platelet adhesion and activation are affected by surface properties of polymers which have been chosen for their potential in biomedical applications, and to relate these responses to their effects on thrombogenicity. The activation of purified human platelets on polymer surfaces, which have been previously characterized by X-ray photoelectron spectroscopy, contact angle, and other techniques; will be studied by Scanning Electron Microscopy, High Voltage Electron Microscopy, and in the living state, by Video-enhanced Differential Interference Contrast Light Microscopy. Differences in cytoskeletal reorganization during shape-change, time and rate of shape-change, appearance and motility of platelet surface receptors, and granule secretion will be analyzed for each surface. Monoclonal antibodies and specific ligands conjugated to colloidal gold will be employed as probes to follow receptor motility by both light and electron microscopy. Differences in shape-change will then be analyzed using pharmacologic agents to modify activation, receptor expression, and cytoskeletal reorganization. These observations will then be related to polymer physicochemical properties. Additionally, three parameters of platelet function which affect thrombogenesis will be analyzed in vitro for the different polymers. These are; platelet dense granule secretion by photoluminescence ATP assay, immuno-gold bead studies of receptor expression, and the extent of activation inducible by platelet agonists. With an iterative process polymers with other surface properties will then be examined. Ultimately, the determination of polymer surface properties which are important in modulating platelet adherence and activation is desired. An understanding of how platelets respond to polymers having different surface properties can provide a basis for developing polymers with minimal adverse effects when placed in the circulation. Knowledge of the mechanisms by which platelets alter their responses to surfaces, as studied through pharmacologic means, could also lead to improved anticoagulant therapies when artificial materials are placed in the circulation. In addition, the observations of receptor appearance and motility, and granule secretion will lead to a better understanding of the platelets' role in modulating thrombosis and to improved in vitro assays for surface thrombogenicity.