PLATELET BIOLOGY

我们将开发细胞的高分辨率标记技术 血小板表面受体和内部结构，使用 胶体金标记法结合相关光和 电子显微镜。VDIC LM、干涉反射比和AIC-LM 活体细胞上胶体金标记受体的示踪将被 与随后的高分辨扫描电子显微镜相关联。蛋白质的高分辨率成像 标记或未标记小金粒的分子将被 用扫描电子显微镜和ATON-DC力显微镜进行了对比研究。我们 将开发涉及高压冷冻机的准备技术， 冷冻干燥、冷冻替代、临界点干燥。 标记是在分子和亚分子水平上的 分辨率使得活性片段和多个表位在一个 可以识别单个分子并查看它们的空间关系 并与细胞表面和内部超微结构相关。

We will develop techniques for high resolution labeling of cell surface receptors and internal structures of platelets, using colloidal gold labeling in conjunction with correlative light and electron microscopy. VDIC LM, interferencelreflectance and AIC-LM tracldng of colloidal gold labeled receptors on living cells will be correlated with subsequent HRSEM. High resolution imaging of protein molecules labeled or unlabeled with small gold particles will be correlatively studied by SEM and aton-dc force microscope (AFM). We will develop preparative techniques involving high-pressure freezer, freeze-drying, freeze substitution, and critical point drying. Labeling is to be at the molecular and submolecular level of resolution such that active fragments and multiple epitopes on a single molecule can be identified and their spatial relationships seen and correlated with cell surface and internal ultrastructure.