PLATELET BIOLOGY

我们将开发高分辨率标记细胞的技术， 血小板的表面受体和内部结构，使用 胶体金标记结合相关光， 电镜 VDIC LM、干涉反射率和AIC-LM 在活细胞上追踪胶体金标记的受体将是 与随后的HRSEM相关。 蛋白质高分辨率成像 标记或未标记小金颗粒的分子将被 用扫描电镜和原子力显微镜（AFM）进行了相关性研究。 我们 将开发包括高压冷冻的制备技术， 冷冻干燥、冷冻置换和临界点干燥。 标记是在分子和亚分子水平上， 分离，使得活性片段和多个表位上的 可以识别单个分子并观察它们的空间关系 与细胞表面和内部超微结构有关。

We will develop techniques for high resolution labeling of cell surface receptors and internal structures of platelets, using colloidal gold labeling in conjunction with correlative light and electron microscopy. VDIC LM, interferencelreflectance and AIC-LM tracldng of colloidal gold labeled receptors on living cells will be correlated with subsequent HRSEM. High resolution imaging of protein molecules labeled or unlabeled with small gold particles will be correlatively studied by SEM and aton-dc force microscope (AFM). We will develop preparative techniques involving high-pressure freezer, freeze-drying, freeze substitution, and critical point drying. Labeling is to be at the molecular and submolecular level of resolution such that active fragments and multiple epitopes on a single molecule can be identified and their spatial relationships seen and correlated with cell surface and internal ultrastructure.