OPTICAL RANGING OF HB/MB IN TISSUES

这项研究计划需要深入发展和量化。 一种无创检测血红蛋白/肌红蛋白的新技术 骨骼肌和心肌中的脱氧作用。一种连续光法 (CWS)基于双波长分光光度原理，可以 直接附着在运动动物或人体的四肢上。 该仪器是一种“趋势指示器”，其校准依据是 有可能出现袖带缺血和充血。光路未知 只有与人类受试者的相对反应才能得到 外周血管疾病或充血性心力衰竭。结果可以 与磷磁共振获得的结果密切相关 光谱学。为了通过直接测量来改善量化 光路，时间分辨光谱(TRS)涉及皮秒或 相位调制光谱(PMS)技术提供了对 单个波长器件的浓度变化和直接 双组分和多组分的浓度及其变化的测量 常氧和缺血肢体的波长动物模型。此外， Hb/Mb的单独贡献将通过1)化学物质进行评估 肌红蛋白的修饰，2)高分辨激光光谱学，3) 血红蛋白信号与微动脉脉搏同步，以及4) 低温下的冷冻捕获和氧化还原扫描。成像 TRS的潜力将在模型、正常动物和肢体中进行研究。 在诱发的间隔室综合征和在模型脑梗塞中 心肌采用单通道CWS和PMS。将PMS和TRS升级到 这些模型的多通道光谱学将提供以下数据集 可以通过适当的算法进行分析，通过该算法可以 可以评估的最终目标是提供一个3-D动画 Hb/Mb在收缩周期中的变化。小鼠的缺氧组织体积 每种模型都将通过冷冻陷阱和3-D氧化还原扫描进行评估 在其他支持下。将尝试通过胸壁进行观察 其中与收缩-舒张期同步可以提供对 心肌组织体积，目的在于提供方法 适用于人类受试者。从而安全、经济、高效 对肌肉缺氧/缺血的评估可能普遍适用于 无需使用大磁铁的普通和专科医学评估 或放射性物质。

This research program calls for an intensive development and quantitation of a new technology for noninvasive measurements of hemoglobin/myoglobin deoxygenation in skeletal and cardiac muscle. A continuous light method (CWS) is base upon the dual wavelength spectrophotometry principle and can be attached directly to the limb of the exercising animal or human subject. This instrument is a "trend indicator" and is calibrated in relation to cuff ischemia and hyperemia where possible. The optical path is unknown and only relative responses are obtained with human subjects with peripheral vascular disease or congestive heart failure. The results can be closely correlated with those obtained by phosphorus magnetic resonance spectroscopy. In order to improve quantitation by direct measurement of the optical path, time resolved spectroscopy (TRS) involving picosecond or phase modulation spectroscopy (PMS) technologies afford a quantitation of concentration changes for a single wavelength device and direct measurements of concentrations and their changes for a dual and multi- wavelength animal models in normoxic and ischemic limbs. Furthermore, the separate contributions of Hb/Mb will be evaluated by 1) chemical modification of myoglobin, 2) highly resolved laser spectroscopy, 3) synchronization of hemoglobin signals with the arteriolar pulse, and 4) freeze trapping and redox scanning at low temperatures. The imaging potential of TRS will be studied in models, in normal animals and in limbs with an induced compartment syndrome and in model infarcts of the myocardium using single channel CWS and PMS. Upgrading PMS and TRS to multichannel spectroscopy of these models will provide the data sets which can be analyzed by appropriate algorithms by which the imaging potential can be evaluated with the ultimate goal providing a 3-D motion picture of Hb/Mb changes over the contraction cycle. The hypoxic tissue volume of each model will be evaluated by freeze trapping and 3-D redox scanning under other support. Observations through the chest wall will be attempted where synchrony with systole-distole may afford identification of myocardial tissue volumes, with an end in view of providing methods applicable to human subjects. Thus safe, economical and efficient evaluation of muscle hypoxia/ischemia may become generally available to general and speciality medical evaluations without the use of large magnets or radioactive materials.