EFFECT OF RETROVIRAL INFECTION ON BONE MARROW

本项目的长期目标是确定 由此逆转录病毒感染影响造血。 鼠 模型系统已经开发，其中WB 91-GV 逆转录病毒感染成年小鼠，导致免疫抑制， 最终是白血病 WB 91-GV与HIV相似， 不表达v-onc基因并且它感染成年动物。 在这些 研究逆转录病毒感染对造血的影响， 发展的时间关系， 将建立免疫缺陷到改变的造血。 的 被WB 91-GV感染的骨髓细胞的类型将是 确定和感染细胞对GM-CSF的反应性， IL 3，以及IL 3加G-CSF、M-CSF或促红细胞生成素的组合 将被评估。 体内治疗感染的效果 在存在或不存在T的情况下用GM-CSF的动物 将检查淋巴细胞。 各种技术， 从体内动物研究到基因表达的分子分析 将用于 这些研究应该能让我们了解 逆转录病毒感染对造血的影响， 如何利用外源性增长因素储备 其中一些影响。

The long-term goal of this project is to determine the mechanisms whereby retroviral infection affects hemopoiesis. A murine model system has been developed in which the WB91-GV retrovirus infects adult mice resulting in immunosuppression and eventually leukemia. WB91-GV is similar to HIV in that it does not express a v-onc gene and it infects adults animals. In these studies the effect of retroviral infection on hemopoiesis will be defined and the temporal relationship of the development of immunodeficiency to altered hemopoiesis will be established. The types of bond marrow cells that are infected by WB91-GV will be determined and the responsiveness of infected cells to GM-CSF, IL3, and combinations of IL3 plus G-CSF, M-CSF or erythropoietin will be assessed. The effect of in vivo treatment of infected animals with GM-CSF in the presence or absence of T lymphocytes will be examined. Diverse technologies ranging from in vivo animal studies to molecular analysis of gene expression will be used. These studies should give us insignt into the effect of retroviral infection on hemopoiesis and should give us experience on how to use exogenous growth factors to reserve some of these effects.