OPTICAL RANGING OF HB/MB IN TISSUES

这项研究计划要求深入的发展和定量 一种新的技术，用于无创测量血红蛋白/肌红蛋白 骨骼肌和心肌的脱氧。 连续光法 (CWS)基于双波长分光光度法原理， 直接附着在运动动物或人体的肢体上。 该工具是一个“趋势指标”，并根据以下因素进行校准： 袖带缺血和充血。 光路未知 并且仅获得了具有以下特征的人类受试者的相对响应： 外周血管疾病或充血性心力衰竭。 研究结果可 与磷磁共振结果密切相关 谱 为了通过直接测量来改善定量， 光路，时间分辨光谱（TRS），涉及皮秒或 相位调制光谱（PMS）技术提供了一个定量的 对于单个波长器件和直接的 测量浓度及其变化的双重和多重- 在常氧和缺血肢体中的波长动物模型。 而且 Hb/Mb的单独贡献将通过1）化学 肌红蛋白的修饰，2）高分辨激光光谱，3） 血红蛋白信号与小动脉脉搏的同步，以及4） 冷冻捕获和低温氧化还原扫描。 成像 将在模型、正常动物和肢体中研究TRS的潜力 与诱导的间隔综合征和模型梗死的 心肌使用单通道CWS和PMS。 升级PMS和TRS， 这些模型的多通道光谱将提供数据集， 可以通过适当的算法进行分析， 最终的目标是提供一个3D电影， Hb/Mb随收缩周期变化。 缺氧组织体积 每个模型都将通过冷冻捕获和三维氧化还原扫描进行评估 在其他支持下。 将尝试通过胸壁进行观察 与收缩-舒张期的同步性可以提供 心肌组织体积，目的在于提供方法， 适用于人体。 从而安全、经济、高效 肌肉缺氧/缺血的评估通常可用于 不使用大型磁体的一般和专业医学评估 或放射性物质。

This research program calls for an intensive development and quantitation of a new technology for noninvasive measurements of hemoglobin/myoglobin deoxygenation in skeletal and cardiac muscle. A continuous light method (CWS) is base upon the dual wavelength spectrophotometry principle and can be attached directly to the limb of the exercising animal or human subject. This instrument is a "trend indicator" and is calibrated in relation to cuff ischemia and hyperemia where possible. The optical path is unknown and only relative responses are obtained with human subjects with peripheral vascular disease or congestive heart failure. The results can be closely correlated with those obtained by phosphorus magnetic resonance spectroscopy. In order to improve quantitation by direct measurement of the optical path, time resolved spectroscopy (TRS) involving picosecond or phase modulation spectroscopy (PMS) technologies afford a quantitation of concentration changes for a single wavelength device and direct measurements of concentrations and their changes for a dual and multi- wavelength animal models in normoxic and ischemic limbs. Furthermore, the separate contributions of Hb/Mb will be evaluated by 1) chemical modification of myoglobin, 2) highly resolved laser spectroscopy, 3) synchronization of hemoglobin signals with the arteriolar pulse, and 4) freeze trapping and redox scanning at low temperatures. The imaging potential of TRS will be studied in models, in normal animals and in limbs with an induced compartment syndrome and in model infarcts of the myocardium using single channel CWS and PMS. Upgrading PMS and TRS to multichannel spectroscopy of these models will provide the data sets which can be analyzed by appropriate algorithms by which the imaging potential can be evaluated with the ultimate goal providing a 3-D motion picture of Hb/Mb changes over the contraction cycle. The hypoxic tissue volume of each model will be evaluated by freeze trapping and 3-D redox scanning under other support. Observations through the chest wall will be attempted where synchrony with systole-distole may afford identification of myocardial tissue volumes, with an end in view of providing methods applicable to human subjects. Thus safe, economical and efficient evaluation of muscle hypoxia/ischemia may become generally available to general and speciality medical evaluations without the use of large magnets or radioactive materials.