EFFECT OF RETROVIRAL INFECTION ON BONE MARROW

这个项目的长期目标是确定机制 因此，逆转录病毒感染会影响造血。一只小鼠 开发了WB91-GV的模型系统 逆转录病毒感染成年小鼠导致免疫抑制和 最终得了白血病。WB91-GV与HIV相似之处在于它 不表达v-onc基因，它会感染成年动物。在这些 研究逆转录病毒感染对造血的影响将是 定义和发展的时间关系 对改变的造血的免疫缺陷将被确立。这个 感染WB91-GV的结合骨髓细胞的类型将是 确定感染细胞对GM-CSF的反应性， IL-3和IL-3加G-CSF、M-CSF或促红细胞生成素的组合 将会被评估。感染的活体治疗效果观察 在T细胞存在或不存在的情况下使用GM-CSF的动物 将对淋巴细胞进行检查。各种技术，从 活体动物实验对基因表达的分子分析 将会被使用。这些研究应该使我们明白这一效果。 逆转录病毒感染对造血的影响，应该给我们 如何利用外源生长因子储备的体会 其中一些影响。

The long-term goal of this project is to determine the mechanisms whereby retroviral infection affects hemopoiesis. A murine model system has been developed in which the WB91-GV retrovirus infects adult mice resulting in immunosuppression and eventually leukemia. WB91-GV is similar to HIV in that it does not express a v-onc gene and it infects adults animals. In these studies the effect of retroviral infection on hemopoiesis will be defined and the temporal relationship of the development of immunodeficiency to altered hemopoiesis will be established. The types of bond marrow cells that are infected by WB91-GV will be determined and the responsiveness of infected cells to GM-CSF, IL3, and combinations of IL3 plus G-CSF, M-CSF or erythropoietin will be assessed. The effect of in vivo treatment of infected animals with GM-CSF in the presence or absence of T lymphocytes will be examined. Diverse technologies ranging from in vivo animal studies to molecular analysis of gene expression will be used. These studies should give us insignt into the effect of retroviral infection on hemopoiesis and should give us experience on how to use exogenous growth factors to reserve some of these effects.