ANALYSIS OF PHARMACOLOGY AND NEURAL CIRCUIT UNDERLYING B

神经生物学研究的一个主要目标是了解 相关神经递质和受体系统的基本组织 神经系统的功能。解决这个问题的一种方法是 研究一个有充分证据的神经回路的突触生理学 从属于特定的行为。该方法已成功应用 对许多人相对简单的神经系统的分析 无脊椎动物。一种在细胞分析中有用的制备方法 海生软体动物赫米森达的行为。神经回路 一个定义明确、量化的行为例子的基础是 使用细胞神经生理学和细胞生物学的组合进行研究 药理技术。使用细胞内记录和刺激 推测运动神经元和接受突触的中间神经元的技术 来自视觉系统的输入将被识别，并对光的反应 这是机密。已识别的踏板神经元和 将对潜在趋光行为的网络的中间神经元进行研究 在完整的动物中，通过 光灭活技术。将对行为的变化进行评估 在选择性地消除神经元中的特定神经元之后 包括特定光感受器类型、中间神经元和推测的电路 运动神经元。介导突触相互作用的神经递质 在这个网络的神经元中将使用药理学方法进行检查 和电生理技术。生物胺的作用，如 多巴胺和5-羟色胺对初级感觉活动的调节 神经元和假定的运动神经元将在分离的神经中进行研究。 系统。由于多肽可能与其他神经递质一起出现， 我们将考察不同多肽对光响应的影响。一个 对神经递质和受体系统的了解 简单神经网络及其对生物胺和生物肽的调节 将为情感性精神障碍的研究提供一个有用的模型系统 更复杂的哺乳动物神经系统。

A major goal of neurobiological research is the understanding of the underlying organization of neurotransmitter and receptor systems related to the function of the nervous system. One approach to this problem is to study the synaptic physiology of a well-documented neural circuit that subserves a specific behavior. This approach has been successfully applied to an analysis of the relative simple nervous systems in many invertebrates. One preparation that has been useful in a cellular analysis of behavior is the marine mollusk Hermissenda. The neural circuit underlying a well-defined and quantified example of behavior will be studied using a combination of cellular neurophysiological and pharmacological techniques. Using intracellular recording and stimulation techniques putative motor neurons and interneurons that receive synaptic input from the visual system will be identified and the response to light classified. The relative contribution of identified pedal neurons and interneurons to the network underlying phototactic behavior will be studied in intact animals after eliminating specific neurons by the photoinactivation technique. Changes in behavior will be assessed following the selective elimination of specific neurons in the neuronal circuit including specific photoreceptor types, interneurons, and putative motor neurons. The neurotransmitters mediating the synaptic interactions among the neurons of this network will be examined using pharmacological and electrophysiological techniques. The role of biogenic amines such as dopamine and serotonin in modulating the activity of primary sensory neurons and putative motor neurons will be studied in the isolated nervous system. Since peptides may occur together with other neurotransmitters, the effects of various peptides on the photoresponse will be examined. An understanding of the neurotransmitter and receptor systems in a relatively simple neural network and its modulation by biogenic amines and peptides will provide a useful model system for studies of affective disorders in more complex mammalian nervous systems.