STRUCTURE AND ORGANIZATION OF THE CEREBRAL CORTEX

建议继续对该细胞的形态、组织、 视皮质神经元的突触联系和免疫组织化学 老鼠、猫和猴子。将使用高尔基-EM技术来检查 局部神经胶质细胞的形态和突触联系 回路神经元和锥体细胞。这些研究的结果将是 与使用推定和潜在抗体获得的数据相关联 神经递质决定化学定义的分布 神经元及其终末的群组。还提出了发展的建议 使用抗体组合的技术，以及抗体加 Golgi-EM来确定差异标记神经元如何相互作用。 虽然长期以来人们认为只有两种形态类型 关于大脑皮层中的突触，这种观点受到了质疑，因为有 当然不止两种类型的轴突终末可以区分 根据它们的突触小泡的大小。它的目的是 确定有多少类型的轴突终末和突触真正存在，如何 它们是分布式的，如果可以在来源之间建立关联 终端，它们的形态和它们的发射器内容。 最后，大鼠的视皮层将被用来确定它是否含有 规则排列的垂直定向神经元组，这给了 皮质是一个有图案的基质。 其目标是更好地定义皮质神经元正常相互作用的方式， 在了解这一点之前，不可能理解这些变化 发生在改变的状态下，以及药物如何改变神经元功能。

It is proposed to continue studies of the morphology, organization, synaptic connections and immunochemistry of neurons in the visual cortices of rat, cat, and monkey. A Golgi-EM technique will be employed to examine the morphology and synaptic connections of neurogliaform cells, local circuit neurons, and pyramidal cells. The results of such studies will be correlated with data obtained using antibodies to putative and potential neurotransmitters to determine the distribution of chemically defined groups of neurons and their terminals. It is also proposed to develop techniques for using combinations of antibodies, and antibodies plus Golgi-EM to determine how differentially labeled neurons interact. Although it has long been held that there are only two morphological types of synapses in cerebral cortex, this view is questioned, for there are certainly more than two types of axon terminals which can be distinguished on the basis of the sizes of their synaptic vesicles. It is intended to ascertain how many types of axon terminals and synapses really exist, how they are distributed, and if correlations can be made between the sources of the terminals, their morphology and their transmitter content. Finally, rat visual cortex will be used to determine if it contains regularly arranged groups of vertically oriented neurons, which give the cortex a patterned matrix. The goal is to better define how cortical neurons normally interact, for until this is understood it is not possible to appreciate the changes which occur in altered states, and how neuronal function can be altered by drugs.