METABOLIC PROCESSES OF THE INNER EAR

这个项目的目的是全面描述新陈代谢 耳蜗区和前庭区的发育过程 内淋巴电位与独特化学物质的维持 内耳不同部位内淋巴的组成。此外， 该项目涉及到听觉的能量和其他方面 Corti和In的Oergan内的转导和神经传递 前庭感觉上皮细胞。我们的目标是通过量化来实现的 几种体外技术用于组织和体液的生化分析 在控制条件下和过程中通过现场电化学方法 或在各种代谢和/或药物干扰之后； 不同代谢状态下的综合电生理评价 干扰，以及由于调查药物或有毒药物的应用 通过血管系统、腔周液或内淋巴应用的药物；通过光和 电子显微镜检查。事实上，在许多情况下，这三个都是 基本方法将结合使用。初级实验 动物将成为小白鼠。因为内耳的复杂性 必须设计非常复杂的技术，其中一些已经 已被开发和实施(例如，耳的动脉灌注 基于人工氧载体的合成血液与 外淋巴灌流；注射调查性药物和毒素 内淋巴)，而另一些则处于不同的发育阶段(如 在不影响电、化学和电的情况下对Scala介质进行灌注 耳蜗功能的形态指标)。超微化学 使用的方法包括酶循环、高效液体 层析、放射免疫分析和氦辉光度法。特价 将努力阐明循环的具体行动模式 氯化物转运时的利尿剂。最终，这些研究应该 更好地了解血管功能不全的影响 并阐明了梅尼埃病的生化基础和 对噪音和耳毒性药物的易感性。

This project is aimed at a comprehensive characterization of the metabolic processes involved in the generation of the cochlear and vestibular endolymphatic potentials and the maintenance of the unique chemical composition of endolymph in different parts of the inner ear. Moreover, the project is concerned with energetic and other aspects of auditory transduction and neural transmission in the oergan of Corti and in vestibular sensory epithelia. Our aims are approached by quantitative biochemical analyses of tissues and fluids by several in vitro techniques and by electrochemical methods in situ under control conditions and during or following various metabolic and/or pharmacological interferences; by comprehensive electrophysiological evaluations during various metabolic interferences, and due to application of investigative drugs or toxic agents applied via the vasculature, perilumph or endolymph; by light and electronmicroscopic evaluations. In fact, in many instances all three basic approaches will be used in combination. The primary experimental animal will be the guinea pig. Because of the complexity of the inner ear very elaborate techniques had to be designed, some of which have already been developed and implemented (e.g. arterial perfusion of the ear with synthetic blood based on artificial oxygen carriers in combination with perilymphatic perfusion; injection of investigative drugs and toxins into endolymph) while others are in various stages of development (e.g. perfusion of scala media without compromising electrical, chemical and morphological indicators of cochlear function). Ultram icrochemical methods used include enzymatic cycling, high performance liquid chromatography, radioimmuno assays and helium glow photometry. Special efforts will be expended to elucidate the specific mode of action of loop dieuretics upon chloride transport. Ultimately, these studies should provide a better understanding of the effects of vascular insufficiency of the ear and shed light upon the biochemical basis of Meniere's disease and the susceptibility to damage by noise and ototoxic drugs.