INNER EAR FLUID DYNAMICS IN HEALTH AND DISEASE

健康和疾病中的内耳流体动力学

• 批准号: 3100136
• 负责人: RUEDIGER R THALMANN
• 金额: $ 58.49万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3100136
• 关键词: fluidity; labyrinth

The major theme of this program project is expressed in our title. Our ultimate goal is to provide a basic foundation for a rational approach to the management of Menjere's disease and other disorders of inner ear fluids. In order to achieve this goal, the fundamental mechanisms underlying fluid dynamics in the inner must first be elucidated. This research, covered in five subprojects, ranges from basic ion transport mechanisms in inner ear epithelia to clinically relevant studies of the adaptive response to interferences with fluid dynamics of the semicircular canals. Project #1 involves the use of a novel tracer technique to measure directly the flow and volume of endolymph in different parts of the inner ear. Project #2 complements project #1 by examining the chemical factors responsible for generation of hydrops and the temporary beneficial effects of glycerol dehydration. In project #3, the most fundamental cellular mechanisms involved in secretion of K and generation of the endolymphatic potential will be studied in the dark cell region of the utricle. Project #4 is aimed at the elucidation of the chemical nature of the tectorial membrane and analogous superstructures of the vestibular system. In addition to a rigorous characterization of the type(s) of collagen in the tectorial membrane and related structures, the amount and distribution of glycosaminoglycans and glycoproteins will be studied by quantitative analytical and special conventional histological preparations of the tectorial membrane.

这个项目的主要主题在我们的标题中表达出来。 我们的最终目标是提供一个基本的基础， 治疗门杰尔病和其他疾病的方法 内耳液体紊乱。 为了实现这一目标， 内部流体动力学的基本机制 必须首先阐明。 这项研究，涵盖在五个 子项目，范围从基本的离子传输机制，在内部 耳上皮细胞的临床相关研究的适应性 对半圆形流体动力学干扰的响应 运河 项目#1涉及使用一种新的示踪技术， 直接测量不同部位内淋巴流量和体积， 内耳的一部分。 项目#2通过以下方式补充项目#1： 检查化学因素负责产生的 水肿和甘油的暂时有益作用 脱水 在项目#3中，最基本的细胞 参与K分泌和产生的机制， 内淋巴电位将在暗细胞区域进行研究， 胞果 项目#4旨在阐明 覆膜和类似物的化学性质 前庭系统的上层结构。 除了严格的 顶盖中胶原类型的表征 膜和相关结构， 糖胺聚糖和糖蛋白的研究将通过 定量分析和特殊常规组织学 覆膜的制备。