BRAINSTEM PROJECTIONS TO CEREBRUM AND CEREBELLUM

描述(研究人员摘要)：试图定义角色的研究 对于它们在发育过程中表达的胶质细胞和独特的分子 那些在创伤期间表达的人将从一个模型系统中受益匪浅 这些结构元素可以很容易地与功能相关 组织。调查员和同事们最近发现 神经胶质细胞和瞬时表达的糖结合物的边界 哺乳动物的大脑发育预测了一种特有的模式- 被称为桶的功能单元，代表单个面部 鼠标中有触须。发育模式的细胞和分子 构筑边界也与存在的突触边界相关联 在出生后晚期和成人脑中。此外，一些相同的 在发育功能单位周围的边界上发现的神经胶质成分 在大脑中也有表达，在受伤的成人大脑中也有表达。现在是时候了 重要的是描述和比较特定的细胞和分子 发育边界和神经胶质“伤疤”的成分，因为其中一个是 显然是预先编程的，另一种是在创伤后诱导的，但两者 可能起到阻止轴突生长的作用。神经胶质细胞的免疫细胞化学研究 和黏附/细胞外基质分子(例如J1/Tenascin和a 可能的蛋白多糖配体)将在胚胎和 皮质和皮质下触觉中心的出生后发育 确定这些重要事件出现和消失的准确时间 发育的细胞和分子决定因素。最早的 组织、发育调节和病变诱导的重组 神经胶质细胞和糖结合物在躯体感觉脑和小脑的分布 大脑皮质将与受控的发育过程进行比较，使用 来自正常脊椎动物的器官类型培养。如果 在正常脑发育过程中观察到的神经胶质/糖共轭边界 具有与那些相关元素相同或不同的元素 创伤后或神经退行性变后成熟脑内的胶质瘢痕 疾病，人们必须对所有这些因素进行分类，并尝试改变 它们的表达是为了促进中枢神经系统的再生。

DESCRIPTION (Investigator's Abstract): Studies attempting to define roles for glia and unique molecules that they express during development versus those expressed during trauma would greatly benefit from a model system where these structural elements could be readily related to functional organization. The investigator and associates have recently discovered boundaries of glia and transiently-expressed glycoconjugates during mammalian brain development that predict a characteristic pattern - functional units referred to as barrels that represent individual facial vibrissae in the mouse. The cells and molecules of developmental, pattern formation boundaries are also associated with synaptic boundaries present in the late postnatal and adult brain. Additionally, some of the same glial constituents found in boundaries around developing functional units in the brain are also expressed in the wounded, adult brain. It is now important to characterize and compare specific cellular and molecular constituents of developmental boundaries and glial "scars", since one is apparently pre-programmed and the other induced following trauma, yet both may function to deter neurite growth. Immunocytochemistry studies of glia and adhesion/extracellular matrix molecules (e.g., J1/tenascin and a possible proteoglycan ligand) will be performed during embryonic and postnatal development of cortical and subcortical vibrissae centers to determine precise times of appearance and disappearance of these important cellular and molecular determinants of development. The early organization, developmental regulation, and lesion-induced reorganization of glia and glycoconjugates in the somatosensory cerebral and cerebellar cortices will be compared with controlled developmental processes using organotypic cultures from normal veruss lesioned animals. If the glial/glycoconjugate boundaries observed during normal brain development possess elements in common with or distinct from those associated with glial scars in the mature brain following trauma or in neurodegenerative disease, one must categorize all of these elements and attempt to alter their expressions in order to facilitate CNS regeneration.