SYNAPTIC ORGANIZATION OF BASAL FOREBRAIN PROJECTIONS

这项研究的目的是详细地建立生理学 成人基底前脑向边缘系统的输入特性 老鼠。将研究三条具体的路径：(1)来自 Broca斜角带至后扣带皮质的核， (2)斜角带向内嗅皮层的投射；(3) 苍白球腹侧核/基底核向基底外侧核的投射 无核的核。这些路径将由 神经生理学技术在活体细胞内记录和 贴标签。电刺激会激活基底前脑， 神经元反应将被记录在靶区的细胞内 区域。扣带回和内嗅觉皮质的靶神经元和 杏仁核将通过标记它们来进行形态识别 细胞内注射辣根过氧化物酶(HRP)。最基本的 丘脑-扣带回突触传递的前脑调制 将对预测进行研究。最终的目标是给基底前脑注射 用HRP对神经元进行细胞内染色，以便详细描述它们的特征 轴突投射和共侧化的模式。 这些研究将建立在体内的生理和形态 颅底广泛投影系统与下颌关节突间的关系 前脑和边缘系统的三个重要组成部分 扣带回皮质(海马区的重要皮质靶点之一 形成)，内嗅觉皮质(输入的主要来源 齿状回和海马体)，以及杏仁核。这些细胞内 因此，研究将填补我们对突触知识的重大空白 这些系统的组织和生理学。

The objective of this research is to establish in detail the physiological properties of inputs from the basal forebrain to the limbic system in adult rats. Three specific pathways will be studies: (1) projections from the nucleus of the diagonal band of Broca to the posterior cingulate cortex, (2) projections from the diagonal band to the entorhinal cortex, and (3) projections from the ventral pallidum/nucleus basalis to the basolateral nucleus of the amgydala. The pathways will be studied by the neurophysiological techniques of in vivo intracellular recording and labeling. The basal forebrain will be activated by electrical stimulation, and neuronal responses will be recorded intracellularly in the target areas. Target neurons in the cingulate and entorhinal cortices and the amygdala will be morphologically identified by labeling them with intracellular injections of horseradish peroxidase (HRP). The basal forebrain modulation of synaptic transmission in thalamo-cingulate projections will be studied. A final goal is to inject basal forebrain neurons intracellularly with HRP in order to characterize in detail their pattern of axonal projections and coilateralization. The studies will establish the in vivo physiological and morphological relationship between the extensive projection system of the basal forebrain, and three important components of the limbic system, the cingulate cortex (one of the important cortical targets of the hippocampal formation), the entorhinal cortex (a principal source of input to the dentate gyrus and hippocampus), and the amygdala. These intracellular studies will thus fill significant gaps in our knowledge about the synaptic organization and physiology of these systems.