SURFACE EVENTS DURING AXONAL GROWTH

我们的目标是阐明一些机制，控制形成 神经元网络在发育和再生过程中的作用。 这些研究 专注于神经生长锥的膜成分所起的作用 在这些机制中。 特别令人感兴趣的是：（一）插入 特定的膜组件，钠通道和生长相关的 蛋白质，进入生长神经元的特定质膜结构域，（ii） 生长锥膜糖蛋白的性质和变异性，（iii） N-CAM样生长相关的 糖蛋白，（iv）膜糖脂的性质和可变性， 生长锥，以及（iv）特定膜成分的外观， 突触形成过程中的神经末梢 拟议的研究利用了 广泛的形态学、生物化学和免疫学技术， 以及组织培养和亚细胞分离。 这项工作是 预计将导致新的见解的分子机制， 生长锥-靶细胞相互作用和突触发生。 这种知识 将成为研究发展和 中枢神经系统退行性疾病和我们对再生的理解 在CNS。

The goal is to elucidate some of the mechanisms that control the formation of neuronal networks during development and regeneration. The studies are focused on the role that membrane components of the nerve growth cone play in these mechanisms. Of particular interest are: (i) the insertion of specific membrane components, the sodium channel and a growth-associated protein, into specific plasmalemmal domains of the growing neuron, (ii) the nature and variability of membrane glycoproteins of the growth cone, (iii) the molecular characteristics of an N-CAM-like, growth-associated glycoprotein, (iv) the nature and variability of membrane glycolipids of the growth cone, and (iv) the appearance of specific membrane components at the nerve terminal during synaptogenesis. The proposed studies make use of a wide range of morphological, biochemical and immunological techniques as well as of tissue culture and subcellular fractionation. This work is expected to lead to new insights into the molecular mechanisms of growth-cone - target-cell interaction and synaptogenesis. Such knowledge will form the basis for studies on the causes of developmental and degenerative disorders of the CNS and for our understanding of regeneration in the CNS.