MOLECULAR ORGANIZATION OF NEURON TERMINALS

脊椎动物突触是细胞间接触的一种特殊形式 在神经系统功能上。质膜及其相关物质 突触前后两侧的胞浆蛋白 形成一个高度专业化、超微结构独特的复合体，即 许多生化和生理调节的细胞内定位 突触事件。 这项拟议的研究旨在检查 神经系统突触前终末。这将由 文档齐全的技术的强大组合：标签 终末内的突触前、亚细胞胞浆室 轴突运输，b)标记突触前终末的丰富 通过仔细和严格地准备突触体，以及c)从属于 从放射性标记的突触小体到生化实验 旨在测试放射性标记蛋白质在体内的功能作用 航站楼。 因此，我们可以通过对正常的基础研究获得信息 突触意志的生物化学、分子组织和功能 帮助a)理解突触前终端执行其 疾病状态应激下的重要生物学任务，以及b)在 辨别影响突触的故障的起源和来源 直接去吧。

The vertebrate synapse is a specialized form of cell-cell contact critical in nervous system function. The plasma membrane and underlying associated cytoplasmic proteins of both the pre- and postsynaptic sides of the synapse form a highly specialized, ultrastructurally unique complex that is the intracellular location of many biochemically and physiologically regulated synaptic events. The proposed study is designed to examine the molecular organization of nervous system presynaptic terminals. This will be accomplished by the powerful combination of well-documented technologies: labelling of the presynaptic, subcellular cytoplasmic compartments within the terminal by axonal transport, b) the enrichment of radiolabeled presynaptic terminals by careful and rigorous preparation of synaptosomes, and c) the subjection of the radiolabeled synaptosomes to biochemical experiments that are designed to test the functional role of the radiolabeled proteins within the terminals. Information we may thus obtain through basic research on normal biochemistry, molecular organization, and functioning of the synapse will aid a) in understanding the way the presynaptic terminal performs its important biological tasks under the stress of disease states, and b) in discerning the origins and source of malfunctions that affect the synapse directly.