MOLECULAR ORGANIZATION OF NEURON TERMINALS

脊椎动物突触是细胞间接触的一种特殊形式 在神经系统功能上。质膜及其相关物质 突触前后两侧的胞浆蛋白 形成高度专业化、超结构独特的复合体，这些复合体是 许多生物化学和生理上的细胞内位置 受调节的突触事件。 这项拟议的研究旨在检查 中央内侧皮质细胞质和相关突触质膜 神经系统突触前终末。这将由 三项记录齐全的技术的强大组合：a)标签 通过轴突在终末内的突触前、亚细胞间隔 运输，b)放射性标记的突触前终末通过 仔细和严格的突触小体的准备和c)服从 这些可放射连接的联会小体进行了一系列实验，类似于 以前用来阐明 富含血影蛋白的细胞骨架及其相关的质膜 红血球。 因此，我们可以通过对正常的基础研究获得信息 突触意志的生物化学、分子组织和功能 辅助A)理解突触前终末执行其 疾病状态应激下的重要生物学任务，以及B)在 辨别影响突触的故障的起源和来源 直接去吧。

The vertebrate synapse is a specialized form of cell-cell contact critical in nervous system function. The plasma membrane and underlying associated cytoplasmic proteins of both the pre- and postsynaptic sides of the synapse form highly specialized, ultrastructurally unique complexes that are the intracellular locations of many of the biochemically and physiologically regulated synaptic events. The proposed study is designed to examine the molecular organization of the cortical cytoplasm and associated synaptic plasma membrane within central nervous system presynaptic terminals. This will be accomplished by the powerful combination of three, well-documented technologies: a) labelling of presynaptic, subcellular compartments within the terminal by axonal transport, b) the enrichment of radiolabeled presynaptic terminals by careful and rigorous preparation of synaptosomes and c) the subjection of these radiolabled synatosomes to a battery of experiments, similar to those previously used to elucidate the structural organization of the spectrin-enriched cytoskeleton and the associated plasma membrane of the red blood cell. Information we may thus obtain through basic research on normal biochemistry, molecular organization, and functioning of the synapse will aid A) in understanding the way the presynaptic terminal performs its important biological tasks under the stress of disease states, and B) in discerning the origins and source of malfunctions that affect the synapse directly.