MOLECULAR ORGANIZATION OF NEURON TERMINALS

神经元末端的分子组织

• 批准号: 3404710
• 负责人: Judy Ann Garner
• 金额: $ 15.01万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3404710
• 关键词: cell membrane; central nervous system; cytoplasm; electron microscopy; gel electrophoresis; injury /disease stressor; microscopy; nerve endings; neuroanatomy; neurochemistry; neuronal transport; neurotransmitters; radioassay; stress; synapses; synaptosomes

The vertebrate synapse is a specialized form of cell-cell contact critical in nervous system function. The plasma membrane and underlying associated cytoplasmic proteins of both the pre-and post synaptic sides of the synapse form a highly specialized, ultrastructurally unique complex that is the intracellular location of many biochemically and physiologically regulated synaptic events. The proposed study is designed to examine the molecular organization of nervous system presynaptic terminals. This will be accomplished by the powerful combination of well- documented technologies: labelling of the presynaptic, subcellular cytoplasmic compartments within the terminal by axonal transport, b) the enrichment of radiolabeled presynaptic terminals by careful and rigorous preparation of synaptosomes, and c) the subjection of the radiolabeled synaptosomes to biochemical experiments that are designed to test the functional role of the radiolabeled proteins within the terminals. Information we may thus obtain through basic research on normal biochemistry, molecular organization, and functioning of the synapse will aid a) in understanding the way the presynaptic terminal performs its important biological tasks under the stress of disease states, and b) in discerning the origins and source of malfunctions that affect the synapse directly.

脊椎动物突触是细胞间的特殊形式 接触对神经系统功能至关重要。 等离子 膜和底层相关的细胞质蛋白 突触的前侧和突触后侧均形成 高度专业化、超微结构独特的复合体 许多生物化学和生理学上的细胞内位置 调节的突触事件。 拟议的研究旨在检查分子 神经系统突触前末梢的组织。 这将 通过良好的强大组合来完成 已记录的技术：突触前的标记， 终端内的亚细胞胞质区室 轴突运输，b）放射性标记突触前的富集 通过仔细和严格的突触体制备来终止， c) 将放射性标记的突触体置于 生化实验旨在测试 放射性标记蛋白质在体内的功能作用 终端。 因此，我们可以通过基础研究获得信息 正常的生物化学、分子组织和功能 突触的研究将有助于 a) 理解突触的方式 突触前末梢执行其重要的生物学任务 在疾病状态的压力下，以及b）辨别 影响突触的故障的起源和根源 直接地。