MOLECULAR ORGANIZATION OF NEURON TERMINALS

脊椎动物突触是细胞的一种特殊形式 在神经系统功能上接触危重病人。血浆 细胞膜及相关细胞质蛋白 突触的突触前和突触后两侧都形成一个 高度专业化、超微结构独特的复合体是 许多生化和生理上的细胞内定位 受调节的突触事件。 这项拟议的研究旨在检查分子 神经系统突触前终末的组织。这将是 通过井与井的强大结合来实现- 记录的技术：突触前的标签， 终末内的亚细胞胞质隔间 轴突运输，b)标记突触前神经元的丰富 通过仔细和严格的突触小体准备终末， 和c)放射性标记的突触体服从 生物化学实验旨在测试 放射性标记蛋白在细胞内的功能作用 终点站。 因此，我们可以通过基础研究获得信息 正常的生物化学、分子组织和功能 将有助于a)理解 突触前终末执行其重要的生物学任务 在疾病状态的压力下，以及b)在辨别 影响突触的故障的起源和来源 直接去吧。

The vertebrate synapse is a specialized form of cell-cell contact critical in nervous system function. The plasma membrane and underlying associated cytoplasmic proteins of both the pre-and post synaptic sides of the synapse form a highly specialized, ultrastructurally unique complex that is the intracellular location of many biochemically and physiologically regulated synaptic events. The proposed study is designed to examine the molecular organization of nervous system presynaptic terminals. This will be accomplished by the powerful combination of well- documented technologies: labelling of the presynaptic, subcellular cytoplasmic compartments within the terminal by axonal transport, b) the enrichment of radiolabeled presynaptic terminals by careful and rigorous preparation of synaptosomes, and c) the subjection of the radiolabeled synaptosomes to biochemical experiments that are designed to test the functional role of the radiolabeled proteins within the terminals. Information we may thus obtain through basic research on normal biochemistry, molecular organization, and functioning of the synapse will aid a) in understanding the way the presynaptic terminal performs its important biological tasks under the stress of disease states, and b) in discerning the origins and source of malfunctions that affect the synapse directly.