METHANOGENIC BACTERIA IN SUBGINGIVAL DENTAL PLAQUE

我们建议研究产甲烷细菌与人类的关系。 牙病。在初步实验中，丰富了培养物和 从各种牙菌斑中获得了分离的菌株 牙周病患者。 长期目标是了解 这部小说中的产甲烷菌，迄今为止还没有被研究过的生态系统，并检查 细菌对人类宿主的影响。这里的具体目标是： (1)确定牙菌斑中是否存在产甲烷菌 主要是在疾病情况下；(2)描述孤立的 使用微生物和免疫学技术的产甲烷菌(3)检查 产甲烷菌调节异养新陈代谢的能力 从牙菌斑获得的混合培养物中的细菌。 龈下菌斑的样本将被厌氧转移到 产甲烷培养基及以H2+CO2为培养基时获得的浓缩培养物 碳和能源。通过平板培养将获得纯培养物。 厌氧培养到凝胶石板上，在H2/CO2下培养。 培养物和纯培养物的免疫学分析以及 营养研究将有助于确定目前存在的产甲烷菌的特征； 初步发现表明，我们可能会发现新的生物体。通过抽样 研究对象广泛，并使用适当的控制和统计方法 我们将能够得出甲烷菌是否与 牙周病。

We propose to study the relationship of methane producing bacteria to human dental disease. In preliminary experiments enrichment cultures and isolated strains have been obtained from dental plaque in a variety of periodontal patients. Long term objectives are to understand the ecological relationships of the methanogens in this novel, heretofore unstudied ecosystem, and to examine the effects of the bacteria on the human host. Specific aims here are to: (1) Determine if the presence of methanogens in dental plaque is principally in cases of diseased conditions; (2) characterize the isolated methanogens using microbiological and immunological techniques (3) examine the ability of methanogens to modify the metabolism of heterotrophic bacteria in mixed cultures obtained from dental plaque. Samples of subgingival plaque will be anaerobically transferred to methanogen medium, and enrichment cultures obtained using H2 + CO2 as a carbon and energy source. Pure cultures will be obtained by plating anaerobically onto Gellrite plates and incubating under H2/CO2. Immunological analysis of enrichments and pure cultures, as well as nutritional studies will allow characterization of the methanogens present; preliminary findings suggest we may discover novel organisms. By sampling a wide range of subjects and using appropriate controls and statistical techniques we will be able to conclude if methanogens are correlated with periodontal disease.