SURFACE EVENTS DURING AXONAL GROWTH

其目的是阐明一些控制形成的机制。 神经网络在发育和再生过程中的变化。这些研究是 重点阐述了神经生长锥膜成分所起的作用 在这些机制中。特别令人感兴趣的是：(1)插入 特定的膜成分，与钠通道和生长相关 蛋白质进入正在生长的神经元的特定的质膜区域，(Ii) 生长锥膜糖蛋白的性质和变异性(III) 一种N-CAM样生长相关分子的分子特征 糖蛋白，(Iv)膜糖脂的性质和变异性 生长锥体，以及(Iv)特定膜组分在 突触发生过程中的神经末梢。拟议的研究利用了 广泛的形态、生化和免疫学技术，如 以及组织培养和亚细胞分离。这项工作是 有望带来对人类免疫缺陷的分子机制的新见解 生长-锥体-靶细胞-细胞相互作用和突触发生。这样的知识 将成为研究发育和肥胖的原因的基础 中枢神经系统退行性疾病与我们对再生的理解 在中枢神经系统。

The goal is to elucidate some of the mechanisms that control the formation of neuronal networks during development and regeneration. The studies are focused on the role that membrane components of the nerve growth cone play in these mechanisms. Of particular interest are: (i) the insertion of specific membrane components, the sodium channel and a growth-associated protein, into specific plasmalemmal domains of the growing neuron, (ii) the nature and variability of membrane glycoproteins of the growth cone, (iii) the molecular characteristics of an N-CAM-like, growth-associated glycoprotein, (iv) the nature and variability of membrane glycolipids of the growth cone, and (iv) the appearance of specific membrane components at the nerve terminal during synaptogenesis. The proposed studies make use of a wide range of morphological, biochemical and immunological techniques as well as of tissue culture and subcellular fractionation. This work is expected to lead to new insights into the molecular mechanisms of growth-cone - target-cell interaction and synaptogenesis. Such knowledge will form the basis for studies on the causes of developmental and degenerative disorders of the CNS and for our understanding of regeneration in the CNS.