STRUCTURE OF ENGINEERED BACTERIOPHAGE PARTICLES

最近的研究已经证明了展示的简单性和实用性 丝状噬菌体表面的外源多肽 应用范围从表位文库的筛选到 疫苗的生产。该项目的目的是确定 各种这些多肽抗原在其上显示时的结构 噬菌体M13的表面。拟议的协作是在 首个报告携带噬菌体产生的实验室 基因8的抗原表位和实验室最近 确定了M13的三维结构。结构研究 将利用工程病毒颗粒的纤维衍射研究来 确定病毒粒子上显示的多肽的构象 浮出水面。对产生的纤维图案的结构分析将是 对原生M13和同构的工作的直接扩展 该病毒的衍生品最近完成了。这些结果将提供 关于抗原性活性结构的重要信息 多肽，因此，应该对设计做出重大贡献 基因工程疫苗。

Recent studies have demonstrated the ease and utility of displaying foreign peptides on the surface of filamentous bacteriophages for applications ranging from screening of epitope libraries to the production of vaccines. This project will be aimed at determining the structure of a variety of these peptide antigens as they are displayed on the surface of bacteriophage M13. The proposed collaboration is between the first laboratory to report the production of bacteriophage carrying antigen epitopes in gene 8 and the laboratory that has recently determined the three-dimensional structure of M13. Structural studies will utilize fiber diffraction studies of engineered virus particles to determine the conformation of the peptides displayed on the virion surface. Structural analysis of the resulting fiber patterns will be a straightforward extension of the work on native M13 and isomorphous derivatives of that virus recently completed. These results will provide important information about the structure of antigenically active peptides and, consequently, should contribute significantly to the design of engineered vaccines.