ENDOGENOUS OPIATE AND MONOAMINE MODULATION OF TORPOR

在生理学和内分泌学方面的信息很少。 每天都麻木。这种体温调节反应可能代表了一种生理性的 处于睡眠和冬眠之间的状态。对传统文化的理解 日常麻木的生理和内分泌基础可能导致 更好地了解睡眠和机制，使逐渐的， 哺乳动物有控制地进入冬眠。 这项提议试图通过计算机化的无线电遥测来表征 Permyscus表现出的诱导(食物配给)麻木模式 马尼特斯，鹿鼠。该提案还寻求确定 内源性阿片类物质，特别是B-内啡肽(BE)，在 调节应激诱导性麻木。有关鸦片类药物的信息将是 通过ip纳洛酮和通过脑室注射获得 在麻木/清醒周期的不同阶段给予BE。 单胺在调节麻木中的作用将通过以下方式确定 使用特定的单胺激动剂和拮抗剂。高效液相色谱分析 下丘脑单胺浓度将被用来表征 这些代谢的时间动力学和功能关系 与麻木有关的单胺类。视交叉上核的作用 (SCN)在规定每日麻木的时间也会 通过使用损伤和阻断单胺代谢来确定 在SCN中。

A paucity of information exists on the physiology and endocrinology of daily torpor. This thermoregulatory response may represent a physiological state intermediate between sleep and hibernation. A understanding of the physiological and endocrinological basis for daily torpor may lead to a better understanding of sleep and the mechanisms that enable the gradual, controlled entry of mammals into hibernation. This proposal seeks to characterize via computerized radiotelemetry the induced (food rationed) torpor patterns displayed by Peromyscus maniculatus, the deermouse. The proposal also seeks to determine the role that the endogenous opiates, in particualr B-endorphin (BE), play in modulating stress induced torpor. Information on the opiates will be obtained through IP administration of naloxone and through intraventricular administration of BE during different stages of the torpor/awake cycle. The role of the monoamines in modulating torpor will be determined through the use of specific monoamine agonists and antagonists. HPLC analyses of hypothalamic monoamine concentrations will be used to characterize the temporal dynamics and functional relationships of the metabolism of these monoamines with respect to torpor. The role of the suprachiasmatic nucleus (SCN) in the regulation of the timing of daily torpor will also be determined through the use of lesions and blockage of monoamine metabolism in the SCN.