PILIN REGULATORY GENES OF PSEUDOMONAS AERUGINOSA

简介(摘自申请者摘要)：铜绿假单胞菌 一种重要的医院内病原体，在囊肿性疾病中引起特别关注 纤维化症患者。对宿主组织的黏附是许多因素之一 参与了这种细菌感染的进展。主修 黏附因子是共同形成菌毛的蛋白质细丝 从细胞表面延伸。人们对其生产情况知之甚少。 铜绿假单胞菌中的菌毛或这一过程是如何调节的。建议数 研究人员将首先通过分离和表征来研究这一领域 不能产生菌毛的调节突变体。的位置。 野生型基因将通过与重组人互补来确定 含铜绿假单胞菌DNA的质粒。这些基因将被测序并 序列分析。基因产物与之相互作用的部位 菌毛蛋白的结构基因将被确定。这样做的具体目的是 项目有(1)分离毛孔蛋白合成调节突变体，(2) 调控基因的精确定位；(3)调控基因的特征 这些基因，以及(4)它们的作用部位的建立 产品。

DESCRIPTION (Adapted from applicant's abstract): Pseudomonas aeruginosa is an important nosocomial pathogen and of special concern among cystic fibrosis patients. Adherence to host tissue is one of many factors involved in the progression of infection with this bacterium. Major adherence factors are the protein filaments collectively forming pili which extend from the cell surface. Little is known concerning the production of pili in P. aeruginosa or how this process is regulated. The proposed research will study this area by first isolating and characterizing regulatory mutants which are unable to produce pili. The location of the wild-type gene will be determined by complementation with recombinant plasmids containing P. aeruginosa DNA. These genes will be sequenced and the sequence analyzed. The site of interaction of the gene products with the pilin structural gene will be determined. The specific aims of this project are (1) the isolation of pilin synthesis regulatory mutants, (2) the precise localization of regulatory genes, (3) the characterization of these genes, and (4) the establishment of a site of action of their products.